Gönüllü Fatma, Ocak Mevlüt Sinan, Dundar Serkan, Özercan İbrahim Hanifi
Isparta Oral and Dental Health Center, Isparta, Turkey.
Firat University, Faculty of Dentistry, Department of Endodontics, Elazıg, Turkey.
J Appl Oral Sci. 2025 Feb 3;33:e20240446. doi: 10.1590/1678-7757-2024-0446. eCollection 2025.
This study evaluated the effect of Stemregen® nutritional supplement on inflammation and resorption in apical periodontitis using a rat model.
Rats were divided in three groups: negative control (n=7), positive control (n=10), and Stemregen® (Stem) (n=10). Apical periodontitis was induced in the positive control and Stem groups, and all rats were sacrificed on the 30th day. Serum phosphorus (P), calcium (Ca), and alkaline phosphatase (ALP) were analyzed. Histopathological assessments measured osteoblastic and osteoclastic activity, inflammation, fibrosis, and abscess density. Immunohistochemical analyses evaluated RANKL, TRAP, and OPG levels.
Results showed significantly lower osteoblastic activity in the negative control compared to Stem and positive control groups (p=0.005). Osteoclastic activity was higher in the positive control (p=0.032). Inflammation and abscess formation were reduced in the Stem group compared to the positive control (p<0.001). OPG levels were lower in the negative control compared to the other groups (p=0.005).
Stemregen® effectively reduced inflammation and bone destruction, suggesting potential benefits for apical periodontitis management, though further research is needed.
本研究使用大鼠模型评估了Stemregen®营养补充剂对根尖周炎炎症和吸收的影响。
将大鼠分为三组：阴性对照组（n = 7）、阳性对照组（n = 10）和Stemregen®（Stem）组（n = 10）。在阳性对照组和Stem组中诱导根尖周炎，所有大鼠在第30天处死。分析血清磷（P）、钙（Ca）和碱性磷酸酶（ALP）。组织病理学评估测量成骨细胞和破骨细胞活性、炎症、纤维化和脓肿密度。免疫组织化学分析评估RANKL、TRAP和OPG水平。
结果显示，与Stem组和阳性对照组相比，阴性对照组的成骨细胞活性显著降低（p = 0.005）。阳性对照组的破骨细胞活性较高（p = 0.032）。与阳性对照组相比，Stem组的炎症和脓肿形成减少（p < 0.001）。与其他组相比，阴性对照组的OPG水平较低（p = 0.005）。
Stemregen®有效减轻了炎症和骨破坏，表明其对根尖周炎治疗可能具有潜在益处，不过仍需进一步研究。
