Qin Li-Juan, Jia Yong-Sen, Zhang Yi-Bing, Wang Yin-Huan
Department of Physiology, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.
College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.
Biomed Rep. 2016 Apr;4(4):444-448. doi: 10.3892/br.2016.613. Epub 2016 Feb 25.
The present study was performed to determine whether aspirin, a cyclooxygenase (COX) inhibitor, has an effect on the expression of connexin 43 (Cx43) in C6 glioma cells. Using an glioma invasion model, the expression of Cx43 protein in C6 cells was significantly increased following aspirin treatment at a dose of 8 mmol/l for 30, 60 and 120 min via western blot analysis. The peak value of the Cx43 expression was observed in C6 cells after 120 min of aspirin treatment, which was significantly reduced by prostaglandin E2 (PGE2). In addition, aspirin also significantly increased the gap junction intercellular communication (GJIC) activity and reduced glioma invasion, which was induced by PGE2. This led to the conclusion that the aspirin-induced glioma invasion decrease may be associated with the increased expression of Cx43 protein and formation of GJIC.
本研究旨在确定环氧化酶（COX）抑制剂阿司匹林是否对C6胶质瘤细胞中连接蛋白43（Cx43）的表达有影响。使用胶质瘤侵袭模型，通过蛋白质印迹分析发现在用8 mmol/l阿司匹林处理30、60和120分钟后，C6细胞中Cx43蛋白的表达显著增加。在阿司匹林处理120分钟后，C6细胞中观察到Cx43表达的峰值，而前列腺素E2（PGE2）可使其显著降低。此外，阿司匹林还显著增加了间隙连接细胞间通讯（GJIC）活性，并减少了由PGE2诱导的胶质瘤侵袭。由此得出结论，阿司匹林诱导的胶质瘤侵袭减少可能与Cx43蛋白表达增加和GJIC的形成有关。
