Sheng Ming, Gorzsás András, Tuck Simon
Umeå Center for Molecular Medicine, Umeå University , Umeå, Sweden.
Department of Chemistry, Umeå University , Umeå, Sweden.
Worm. 2016 Feb 18;5(1):e1132978. doi: 10.1080/21624054.2015.1132978. eCollection 2016 Jan-Mar.
Changes in intermediary metabolism have profound effects on many aspects of C. elegans biology including growth, development and behavior. However, many traditional biochemical techniques for analyzing chemical composition require relatively large amounts of starting material precluding the analysis of mutants that cannot be grown in large amounts as homozygotes. Here we describe a technique for detecting changes in the chemical compositions of C. elegans worms by Fourier transform infrared microspectroscopy. We demonstrate that the technique can be used to detect changes in the relative levels of carbohydrates, proteins and lipids in one and the same worm. We suggest that Fourier transform infrared microspectroscopy represents a useful addition to the arsenal of techniques for metabolic studies of C. elegans worms.
中间代谢的变化对秀丽隐杆线虫生物学的许多方面都有深远影响，包括生长、发育和行为。然而，许多用于分析化学成分的传统生化技术需要相对大量的起始材料，这使得无法大量培养纯合子的突变体分析受到限制。在此，我们描述了一种通过傅里叶变换红外显微光谱法检测秀丽隐杆线虫化学成分变化的技术。我们证明该技术可用于检测同一条线虫中碳水化合物、蛋白质和脂质相对水平的变化。我们认为傅里叶变换红外显微光谱法是秀丽隐杆线虫代谢研究技术库中的一项有用补充。
