小麦胚凝集素与中国仓鼠卵巢细胞凝集素抗性突变体结合的选择性丧失。
Selective loss of wheat germ agglutinin binding to agglutinin-resistant mutants of Chinese hamster ovary cells.
作者信息
Stanley P, Carver J P
出版信息
Proc Natl Acad Sci U S A. 1977 Nov;74(11):5056-9. doi: 10.1073/pnas.74.11.5056.
Abstract
The binding of 125I-labeled wheat germ agglutinin (WGA) to parental and three distinct WGA-resistant Chinese hamster ovary cell lines possessing modified cell surface carbohydrate structures has been examined over a 10(6)-fold range of WGA concentrations. The Scatchard plot for WGA binding to parental cells was complex and exhibited positively cooperative binding at the high affinity sites. One of the WGA-resistant mutants (WgaRIII) was apparently not altered in its WGA-binding ability compared with parental cells. However, two of the WGA-resistant lines (WgaRI and WgaRII) had distinct alterations in their WGA-binding properties specific to certain regions of the binding curve. Neither appeared to be affected in either the highest or lowest affinity regions of the binding curve. Thus, lectin-resistant cell mutants altered in specific lectin-binding sites at the cell surface provide a direct approach to analysis of the complex binding parameters that characterize the interaction of WGA with the plasma membrane.
摘要
在10⁶倍的麦胚凝集素（WGA）浓度范围内，研究了¹²⁵I标记的WGA与亲本细胞以及三种具有修饰细胞表面碳水化合物结构的不同WGA抗性中国仓鼠卵巢细胞系的结合情况。WGA与亲本细胞结合的Scatchard图很复杂，在高亲和力位点表现出正协同结合。其中一个WGA抗性突变体（WgaRIII）与亲本细胞相比，其WGA结合能力显然没有改变。然而，两个WGA抗性细胞系（WgaRI和WgaRII）在结合曲线的特定区域，其WGA结合特性有明显改变。在结合曲线的最高或最低亲和力区域，两者似乎都没有受到影响。因此，细胞表面特定凝集素结合位点发生改变的凝集素抗性细胞突变体，为分析表征WGA与质膜相互作用的复杂结合参数提供了一种直接方法。
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