Treangen Todd J, Schoeler George, Phillippy Adam M, Bergman Nicholas H, Turell Michael J
National Biodefense Analysis and Countermeasures Center, Frederick, Maryland, United States of America.
Department of Entomology, U. S. Naval Medical Research Unit No. 6, Callao, Peru.
PLoS Negl Trop Dis. 2016 Apr 13;10(4):e0004440. doi: 10.1371/journal.pntd.0004440. eCollection 2016 Apr.
Group C orthobunyaviruses are single-stranded RNA viruses found in both South and North America. Until very recently, and despite their status as important vector-borne human pathogens, no Group C whole genome sequences containing all three segments were available in public databases. Here we report a Group C orthobunyavirus, named El Huayo virus, isolated from a pool of Culex portesi mosquitoes captured near Iquitos, Peru. Although initial metagenomic analysis yielded only a handful of reads belonging to the genus Orthobunyavirus, single contig assemblies were generated for L, M, and S segments totaling over 200,000 reads (~0.5% of sample). Given the moderately high viremia in hamsters (>107 plaque-forming units/ml) and the propensity for Cx. portesi to feed on rodents, it is possible that El Huayo virus is maintained in nature in a Culex portesi/rodent cycle. El Huayo virus was found to be most similar to Peruvian Caraparu virus isolates and constitutes a novel subclade within Group C.
C组正布尼亚病毒是在南美洲和北美洲均有发现的单链RNA病毒。直到最近，尽管它们作为重要的媒介传播的人类病原体，但公共数据库中尚无包含所有三个片段的C组全基因组序列。在此，我们报告一种从秘鲁伊基托斯附近捕获的波氏库蚊群体中分离出的C组正布尼亚病毒，命名为埃尔瓦约病毒。尽管最初的宏基因组分析仅产生了少数属于正布尼亚病毒属的读数，但针对L、M和S片段生成了单重叠群组装，总计超过200,000个读数（约占样本的0.5%）。鉴于仓鼠中病毒血症水平中等偏高（>107 空斑形成单位/毫升）以及波氏库蚊有吸食啮齿动物血液的倾向，埃尔瓦约病毒有可能在自然界中通过波氏库蚊/啮齿动物循环得以维持。发现埃尔瓦约病毒与秘鲁卡拉帕鲁病毒分离株最为相似，并在C组内构成一个新的亚分支。
