新鲜和冷冻保存胚胎的视频摄影术：胚胎形态与着床的回顾性分析

Videocinematography of fresh and cryopreserved embryos: a retrospective analysis of embryonic morphology and implantation.

作者信息

Cohen J, Inge K L, Suzman M, Wiker S R, Wright G

机构信息

Reproductive Biology Associates, Atlanta, Georgia.

出版信息

Fertil Steril. 1989 May;51(5):820-7. doi: 10.1016/s0015-0282(16)60673-8.

DOI:10.1016/s0015-0282(16)60673-8

PMID:2707457

Abstract

Eleven morphologic criteria were studied from videotapes of 323 fresh and 103 thawed embryos. The proportion of thawed embryos (30/39; 77%) with more than one abnormality was (P = 0.03) higher than that of fresh embryos (5/13; 38%), despite similar implantation rates (18% and 15%, respectively). The best predictor of thawed embryo implantation was cell-cell adherence: when scored "positive," 11 of 17 women (65%) became pregnant, whereas none became pregnant (0/15; P = 0.0002) when blastomeres did not adhere. The total number of abnormalities for thawed embryos was an important prognostic. An increased percentage variation of zona pellucida thickness was the most important prognostic for fresh embryos. When the "best" embryo had a zona pellucida that varied more than 25%, 24 of 60 (40%) resulted in pregnancy; pregnancies were not induced (0/21) when the "best" embryo had less than 10% variation (P = 0.0003).

摘要

从323个新鲜胚胎和103个解冻胚胎的录像带中研究了11种形态学标准。尽管植入率相似（分别为18%和15%），但解冻胚胎中存在一种以上异常的比例（30/39；77%）高于新鲜胚胎（5/13；38%）（P = 0.03）。解冻胚胎植入的最佳预测指标是细胞间黏附：评分为“阳性”时，17名女性中有11名（65%）怀孕，而当卵裂球不黏附时，无人怀孕（0/15；P = 0.0002）。解冻胚胎异常的总数是一个重要的预后指标。透明带厚度百分比变化增加是新鲜胚胎最重要的预后指标。当“最佳”胚胎的透明带变化超过25%时，60个中有24个（40%）导致怀孕；当“最佳”胚胎的变化小于10%时，未诱导出怀孕（0/21）（P = 0.0003）。