Cohen J, Wiemer K E, Wright G
Department of Obstetrics and Gynecology, Emory University, Atlanta, Georgia.
Fertil Steril. 1988 May;49(5):827-34. doi: 10.1016/s0015-0282(16)59892-6.
Fifty embryos, previously frozen at the zygote or 2- to 4-cell stage, were studied. Observers, who were unaware of the occurrence of pregnancy, assessed the morphologic features of these embryos by retrospectively analyzing videocinematographic recordings that were made shortly before replacement. Seven of 26 zygotes and 4 of 24 cleaved embryos implanted, with the incidence of implantation being analyzed in relation to 12 morphologic characteristics. Smooth surface of the blastomere's membranes was a statistically significant predictor of implantation for frozen-cleaved embryos, and variable zona pellucida thickness was the only parameter with predictive value for frozen zygotes. A highly significant difference was found between the implanting capacity of previously frozen-cleaved embryos and the number of abnormal morphologic characteristics. Three quarters of the thawed embryos had at least two abnormal characteristics, indicating that cryodamage was high. Major advantages of videocinematography are the absence of time constraints associated with observing live embryos, the ability to observe new morphologic parameters by freeze-frame and slow motion, and the permanent storage of embryonic data for quality control evaluation.
研究了50个先前在合子期或2至4细胞期冷冻的胚胎。观察者在不知道妊娠情况的前提下，通过回顾性分析移植前不久拍摄的视频电影记录来评估这些胚胎的形态特征。26个合子中有7个、24个分裂胚胎中有4个着床，并分析了着床发生率与12种形态特征的关系。卵裂球膜表面光滑是冷冻分裂胚胎着床的统计学显著预测指标，透明带厚度可变是冷冻合子唯一具有预测价值的参数。发现先前冷冻分裂胚胎的着床能力与异常形态特征数量之间存在高度显著差异。四分之三的解冻胚胎至少有两个异常特征，表明冷冻损伤程度很高。视频电影摄影的主要优点是观察活胚胎时没有时间限制，能够通过定格和慢动作观察新的形态参数，以及永久存储胚胎数据用于质量控制评估。
