Wang Ting, Wang Ning
School of Pharmacy, Anhui Medical University, 81 Plum Hill Road, Hefei, Anhui Province, 230032, China.
School of Medical Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui Province, 230009, China.
Methods Mol Biol. 2016;1404:651-667. doi: 10.1007/978-1-4939-3389-1_42.
Recently, the multifunctional liposome-constituted microneedle arrays (LiposoMAs) have been proven to be an interesting vaccine adjuvant-delivery system (VADS) that are stable and can be vaccinated via oral cavity mucosal route. When given to mice at oral mucosa, the LiposoMAs can effectively eliminate the ingredient loss caused by chewing, swallowing, and saliva flowing and can, thus, elicit robust systemic as well as mucosal immunoresponses against the loaded antigens. In addition, the LiposoMAs can induce a mixed Th1/Th2 immunoresponse and strong cellular/humoral immunity due to special adjuvanticity and targeting delivery functions of the nanoparticulate VADS. In this chapter, the preparation, characterization as well as mucosal vaccination of the LiposoMAs are introduced. In addition, the methods for sampling mouse organs, tissues, and cells and for evaluation of the immunization efficacy are mainly included.
最近，已证实多功能脂质体构成的微针阵列（LiposoMAs）是一种有趣的疫苗佐剂递送系统（VADS），该系统稳定且可通过口腔黏膜途径进行接种。当在小鼠口腔黏膜给药时，LiposoMAs能有效消除因咀嚼、吞咽和唾液流动导致的成分损失，因此能够引发针对负载抗原的强大全身及黏膜免疫反应。此外，由于纳米颗粒VADS具有特殊的佐剂性和靶向递送功能，LiposoMAs可诱导Th1/Th2混合免疫反应以及强大的细胞/体液免疫。在本章中，将介绍LiposoMAs的制备、表征以及黏膜接种。此外，还主要包括小鼠器官、组织和细胞的采样方法以及免疫效果评估方法。
