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本文引用的文献

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Detection of carbapenemase activity using VITEK MS: interplay of carbapenemase type and period of incubation.使用VITEK MS检测碳青霉烯酶活性：碳青霉烯酶类型与孵育时间的相互作用
J Med Microbiol. 2015 Aug;64(8):946-947. doi: 10.1099/jmm.0.000102.
2
Characterization of BKC-1 class A carbapenemase from Klebsiella pneumoniae clinical isolates in Brazil.巴西肺炎克雷伯菌临床分离株中BKC-1 A类碳青霉烯酶的特性分析
Antimicrob Agents Chemother. 2015 Sep;59(9):5159-64. doi: 10.1128/AAC.00158-15. Epub 2015 Jun 8.
3
Detection of carbapenemase activity directly from blood culture vials using MALDI-TOF MS: a quick answer for the right decision.使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）直接从血培养瓶中检测碳青霉烯酶活性：为正确决策提供快速答案。
J Antimicrob Chemother. 2014 Aug;69(8):2132-6. doi: 10.1093/jac/dku094. Epub 2014 Apr 9.
4
Impact of the isolation medium for detection of carbapenemase-producing Enterobacteriaceae using an updated version of the Carba NP test.使用改良版Carba NP试验检测产碳青霉烯酶肠杆菌科细菌时，分离培养基的影响
J Med Microbiol. 2014 May;63(Pt 5):772-776. doi: 10.1099/jmm.0.071340-0. Epub 2014 Mar 3.
5
Detection of SPM-1-producing Pseudomonas aeruginosa and class D β-lactamase-producing Acinetobacter baumannii isolates by use of liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization-time of flight mass spectrometry.应用液相色谱-质谱联用技术和基质辅助激光解吸电离飞行时间质谱法检测产 SPM-1 型金属β-内酰胺酶的铜绿假单胞菌和产 D 类β-内酰胺酶的鲍曼不动杆菌。
J Clin Microbiol. 2013 Jan;51(1):287-90. doi: 10.1128/JCM.02365-12. Epub 2012 Oct 24.
6
Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based functional assay for rapid detection of resistance against β-lactam antibiotics.基于基质辅助激光解吸电离飞行时间质谱的功能测定法快速检测β-内酰胺类抗生素耐药性。
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8
Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours.利用基质辅助激光解吸电离飞行时间质谱在 1 至 2.5 小时内检测碳青霉烯类耐药性。
J Clin Microbiol. 2011 Sep;49(9):3321-4. doi: 10.1128/JCM.00287-11. Epub 2011 Jul 27.
9
Carbapenemases: the versatile beta-lactamases.碳青霉烯酶：多功能β-内酰胺酶
Clin Microbiol Rev. 2007 Jul;20(3):440-58, table of contents. doi: 10.1128/CMR.00001-07.

培养基对基质辅助激光解吸电离飞行时间质谱法检测碳青霉烯水解的影响

Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.

作者信息

Ramos Ana Carolina, Carvalhaes Cecília Godoy, Cordeiro-Moura Jhonatha Rodrigo, Rockstroh Anna Carolina, Machado Antonia Maria Oliveira, Gales Ana Cristina

机构信息

Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil

Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil.

出版信息

J Clin Microbiol. 2016 Jul;54(7):1896-1898. doi: 10.1128/JCM.00749-16. Epub 2016 Apr 13.

DOI:10.1128/JCM.00749-16
PMID:27076665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4922090/
Abstract

In this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. False-negative results were observed for OXA-25-, OXA-26-, and OXA-72-producing Acinetobacter baumannii isolates grown on MacConkey agar medium. The other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase.

摘要

在本研究中，我们评估了不同细菌生长培养基对采用基质辅助激光解吸电离飞行时间质谱法检测碳青霉烯酶水解的影响。在麦康凯琼脂培养基上生长的产OXA-25、OXA-26和OXA-72的鲍曼不动杆菌分离株出现了假阴性结果。其他培养基对检测碳青霉烯酶显示出100%的敏感性和100%的特异性。