• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

地榆中没食子酸、鞣花酸和槲皮素的定量分析及体外抗炎作用

Quantitative Analysis and In vitro Anti-inflammatory Effects of Gallic Acid, Ellagic Acid, and Quercetin from Radix Sanguisorbae.

作者信息

Seo Chang-Seob, Jeong Soo-Jin, Yoo Sae-Rom, Lee Na-Ri, Shin Hyeun-Kyoo

机构信息

K-Herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of Korea.

KM Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-g u, Daejeon 34054, Republic of Korea.

出版信息

Pharmacogn Mag. 2016 Apr-Jun;12(46):104-8. doi: 10.4103/0973-1296.177908.

DOI:10.4103/0973-1296.177908
PMID:27076745
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4809163/
Abstract

BACKGROUND

Radix Sanguisorbae has long been used to treat diarrhea, enteritis, duodenal ulcers, and internal hemorrhage.

OBJECTIVE

We investigated the in vitro anti-inflammatory effects of Radix Sanguisorbae and performed quantitative analyses of three marker components, namely gallic acid, ellagic acid, and quercetin, using high-performance liquid chromatography coupled with a photodiode array detector.

MATERIALS AND METHODS

The three marker components were separated using a reversed-phase Gemini C18 analytical column maintained at 40°C by the gradient elution with two solvent systems. We examined the biological effects of the three marker compounds, gallic acid, ellagic acid, and quercetin, by determining their anti-inflammatory activities in the murine macrophage cell line RAW 264.7.

RESULTS

All of the marker compounds exhibited inhibitory effects on prostaglandin E2 production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, with no cytotoxicity. Particularly, ellagic acid significantly inhibited production of the proinflammatory cytokines tumor necrosis factor alpha and interleukin-6 in LPS-treated RAW 264.7 cells.

CONCLUSION

Our results suggest that ellagic acid is the most potent bioactive phytochemical component of radix Sanguisorbae in the treatment of inflammatory diseases.

SUMMARY

Established high-performance liquid chromatography method was applied in the quantitative analysis of gallic acid, ellagic acid, and quercetin present in an extract from radix SanguisorbaeAmong the three compounds, the ellagic acid.(7.65.mg/g) is main component in radix SanguisorbaeEllagic acid significantly inhibited production of the proinflammatory cytokines tumor necrosis factor alpha and interleukin-6 in lipopolysaccharide-treated RAW 264.7 cells. Abbreviations used: HPLC: High-performance liquid chromatography, PDA: Photodiode array, TNF-α: Tumor necrosis factor alpha, IL: Interleukin, LPS: Lipopolysaccharide, PGE2: Prostaglandin E2, NSAIDs: Nonsteroidal anti-inflammatory drugs, COX: Cyclooxygenase.

摘要

背景

地榆长期以来一直用于治疗腹泻、肠炎、十二指肠溃疡和内出血。

目的

我们研究了地榆的体外抗炎作用，并使用高效液相色谱结合光电二极管阵列检测器对三种标记成分，即没食子酸、鞣花酸和槲皮素进行了定量分析。

材料与方法

使用反相Gemini C18分析柱，通过两种溶剂系统的梯度洗脱在40°C下分离三种标记成分。我们通过测定没食子酸、鞣花酸和槲皮素这三种标记化合物在小鼠巨噬细胞系RAW 264.7中的抗炎活性，研究了它们的生物学效应。

结果

所有标记化合物对脂多糖（LPS）刺激的RAW 264.7巨噬细胞中前列腺素E2的产生均表现出抑制作用，且无细胞毒性。特别是，鞣花酸显著抑制LPS处理的RAW 264.7细胞中促炎细胞因子肿瘤坏死因子α和白细胞介素-6的产生。

结论

我们的结果表明，鞣花酸是地榆治疗炎症性疾病中最有效的生物活性植物化学成分。

总结

已建立的高效液相色谱法用于地榆提取物中没食子酸、鞣花酸和槲皮素的定量分析。在这三种化合物中，鞣花酸（7.65mg/g）是地榆中的主要成分。鞣花酸显著抑制脂多糖处理的RAW 264.7细胞中促炎细胞因子肿瘤坏死因子α和白细胞介素-6的产生。使用的缩写：HPLC：高效液相色谱，PDA：光电二极管阵列，TNF-α：肿瘤坏死因子α，IL：白细胞介素，LPS：脂多糖，PGE2：前列腺素E2，NSAIDs：非甾体抗炎药，COX：环氧化酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/9d5f0ee39b4b/PM-12-104-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/ab5380a24251/PM-12-104-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/446c65ec7466/PM-12-104-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/cbf5942728ec/PM-12-104-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/309418dfa121/PM-12-104-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/ddd71bb6e0a4/PM-12-104-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/55dc95e5746d/PM-12-104-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/9d5f0ee39b4b/PM-12-104-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/ab5380a24251/PM-12-104-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/446c65ec7466/PM-12-104-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/cbf5942728ec/PM-12-104-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/309418dfa121/PM-12-104-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/ddd71bb6e0a4/PM-12-104-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/55dc95e5746d/PM-12-104-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/524a/4809163/9d5f0ee39b4b/PM-12-104-g007.jpg

相似文献

1
Quantitative Analysis and In vitro Anti-inflammatory Effects of Gallic Acid, Ellagic Acid, and Quercetin from Radix Sanguisorbae.地榆中没食子酸、鞣花酸和槲皮素的定量分析及体外抗炎作用
Pharmacogn Mag. 2016 Apr-Jun;12(46):104-8. doi: 10.4103/0973-1296.177908.
2
Anti-inflammatory potential of ellagic acid, gallic acid and punicalagin A&B isolated from Punica granatum.从石榴中分离出的鞣花酸、没食子酸和石榴皮素A&B的抗炎潜力。
BMC Complement Altern Med. 2017 Jan 14;17(1):47. doi: 10.1186/s12906-017-1555-0.
3
Quantification Analysis and Anti-Inflammatory Effects of 20-Hydroxyecdysone, Momordin Ic, and Oleanolic Acid from the Fructus of .来自[果实名称未给出]果实的20-羟基蜕皮甾酮、苦瓜素Ic和齐墩果酸的定量分析及抗炎作用
Pharmacogn Mag. 2017 Jul-Sep;13(51):339-344. doi: 10.4103/0973-1296.211023. Epub 2017 Jul 19.
4
Phytochemical Analysis on Quantification and the Inhibitory Effects on Inflammatory Responses from the Fruit of Xanthii fructus.苍耳子果实的植物化学分析：定量及对炎症反应的抑制作用
Pharmacogn Mag. 2015 Oct;11(Suppl 4):S585-91. doi: 10.4103/0973-1296.172966.
5
Evaluation of anti-inflammatory and mechanism of action of extract of Macrosiphonia longiflora (Desf.) Müll. Arg.长花大蚜提取物的抗炎作用及其作用机制评估
J Ethnopharmacol. 2014 Jun 11;154(2):319-29. doi: 10.1016/j.jep.2014.03.017. Epub 2014 Mar 25.
6
Anti-inflammatory effects of Phyllanthus amarus Schum. & Thonn. through inhibition of NF-κB, MAPK, and PI3K-Akt signaling pathways in LPS-induced human macrophages.叶下珠通过抑制 LPS 诱导的人巨噬细胞中的 NF-κB、MAPK 和 PI3K-Akt 信号通路发挥抗炎作用。
BMC Complement Altern Med. 2018 Jul 25;18(1):224. doi: 10.1186/s12906-018-2289-3.
7
Ethanol extract from a Chinese herbal formula, "Zuojin Pill", inhibit the expression of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 mouse macrophages.一种中药方剂“左金丸”的乙醇提取物可抑制脂多糖刺激的 RAW 264.7 小鼠巨噬细胞中炎症介质的表达。
J Ethnopharmacol. 2012 May 7;141(1):377-85. doi: 10.1016/j.jep.2012.02.049. Epub 2012 Mar 6.
8
Inhibition of Pro-inflammatory Mediators and Cytokines by Chlorella Vulgaris Extracts.小球藻提取物对促炎介质和细胞因子的抑制作用。
Pharmacognosy Res. 2016 Apr-Jun;8(2):118-22. doi: 10.4103/0974-8490.172660.
9
[Charcoal-frying process and quality markers of Sanguisorbae Radix based on hemostatic effect].基于止血作用的地榆炭炮制工艺及质量标志物研究
Zhongguo Zhong Yao Za Zhi. 2024 May;49(10):2710-2721. doi: 10.19540/j.cnki.cjcmm.20240110.301.
10
Effects of compounds from bi-qi capsule on the expression of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 macrophages.芪倍胶囊含药血清对脂多糖诱导的 RAW264.7 巨噬细胞炎症介质表达的影响。
J Ethnopharmacol. 2011 Jul 14;136(3):480-7. doi: 10.1016/j.jep.2010.06.008. Epub 2010 Jun 15.

引用本文的文献

1
Investigation of In Vitro and In Silico Anti-Inflammatory Potential of L. Root Juice.L.根汁的体外和计算机模拟抗炎潜力研究
Int J Mol Sci. 2025 Jun 21;26(13):5965. doi: 10.3390/ijms26135965.
2
Therapeutic Potential of Ellagic Acid in Liver Diseases.鞣花酸在肝脏疾病中的治疗潜力
Molecules. 2025 Jun 15;30(12):2596. doi: 10.3390/molecules30122596.
3
In vitro study of the treatment of dentin hypersensitivity with gallic acid combined with sodium fluoride.体外研究没食子酸联合氟化钠治疗牙本质敏感症。

本文引用的文献

1
Multi-target approach for natural products in inflammation.天然产物在炎症治疗中的多靶点作用方法
Drug Discov Today. 2014 Dec;19(12):1871-82. doi: 10.1016/j.drudis.2014.08.006. Epub 2014 Aug 27.
2
Eruca sativa and its flavonoid components, quercetin and isorhamnetin, improve skin barrier function by activation of peroxisome proliferator-activated receptor (PPAR)-α and suppression of inflammatory cytokines.芝麻菜及其类黄酮成分槲皮素和山柰酚通过激活过氧化物酶体增殖物激活受体 (PPAR)-α 和抑制炎症细胞因子来改善皮肤屏障功能。
Phytother Res. 2014 Sep;28(9):1359-66. doi: 10.1002/ptr.5138. Epub 2014 Mar 9.
3
Desoxyrhapontigenin, a potent anti-inflammatory phytochemical, inhibits LPS-induced inflammatory responses via suppressing NF-κB and MAPK pathways in RAW 264.7 cells.
BMC Oral Health. 2024 Oct 30;24(1):1319. doi: 10.1186/s12903-024-05098-5.
4
The Potential Health Benefits of Gallic Acid: Therapeutic and Food Applications.没食子酸对健康的潜在益处：治疗及食品应用
Antioxidants (Basel). 2024 Aug 18;13(8):1001. doi: 10.3390/antiox13081001.
5
Unlocking the Therapeutic Potential of Ellagic Acid for Non-Alcoholic Fatty Liver Disease and Non-Alcoholic Steatohepatitis.解锁鞣花酸对非酒精性脂肪性肝病和非酒精性脂肪性肝炎的治疗潜力。
Antioxidants (Basel). 2024 Apr 18;13(4):485. doi: 10.3390/antiox13040485.
6
Anti-Inflammatory and Anti-Adipocyte Dysfunction Effects of Latex and Root Extracts.乳胶和根提取物的抗炎及抗脂肪细胞功能障碍作用
Pharmaceuticals (Basel). 2024 Feb 23;17(3):287. doi: 10.3390/ph17030287.
7
Fruit Extract Alleviates DSS-Induced Ulcerative Colitis by Regulating Gut Microbiota, Inflammatory Mediators, and Cytokines.水果提取物通过调节肠道微生物群、炎症介质和细胞因子缓解 DSS 诱导的溃疡性结肠炎。
Molecules. 2023 Jul 31;28(15):5783. doi: 10.3390/molecules28155783.
8
Olive Oil as a Transport Medium for Bioactive Molecules of Plants?-An In Situ Study.橄榄油作为植物生物活性分子的传递介质？——原位研究。
Molecules. 2023 Apr 28;28(9):3803. doi: 10.3390/molecules28093803.
9
Ellagic Acid and Its Metabolites as Potent and Selective Allosteric Inhibitors of Liver Pyruvate Kinase.鞣花酸及其代谢物作为肝丙酮酸激酶的有效和选择性别构抑制剂。
Nutrients. 2023 Jan 22;15(3):577. doi: 10.3390/nu15030577.
10
Regulating Th17/Treg Balance Contributes to the Therapeutic Effect of Ziyuglycoside I on Collagen-Induced Arthritis.调节 Th17/Treg 平衡有助于紫薇总苷 I 对胶原诱导性关节炎的治疗作用。
Int J Mol Sci. 2022 Dec 17;23(24):16105. doi: 10.3390/ijms232416105.
去甲二氢愈创木酸，一种有效的抗炎植物化学物质，通过抑制 RAW 264.7 细胞中的 NF-κB 和 MAPK 通路来抑制 LPS 诱导的炎症反应。
Int Immunopharmacol. 2014 Jan;18(1):182-90. doi: 10.1016/j.intimp.2013.11.022. Epub 2013 Dec 2.
4
Inhibition of lipopolysaccharide-induced proinflammatory responses by Buddleja officinalis extract in BV-2 microglial cells via negative regulation of NF-kB and ERK1/2 signaling.密蒙花提取物通过负调控 NF-κB 和 ERK1/2 信号通路抑制 BV-2 小胶质细胞中脂多糖诱导的促炎反应。
Molecules. 2013 Jul 31;18(8):9195-206. doi: 10.3390/molecules18089195.
5
Topical anti-inflammatory and analgesic activities of standardized pomegranate rind extract in comparison with its marker compound ellagic acid in vivo.标准化石榴皮提取物与标志物化合物鞣花酸的体内抗炎和镇痛活性比较。
J Ethnopharmacol. 2013 Jul 30;148(3):901-8. doi: 10.1016/j.jep.2013.05.040. Epub 2013 Jun 3.
6
Anti-inflammatory and anti-allergic activities of Pentaherb formula, Moutan Cortex (Danpi) and gallic acid.五草方、牡丹皮（丹皮）和没食子酸的抗炎和抗过敏活性。
Molecules. 2013 Feb 25;18(3):2483-500. doi: 10.3390/molecules18032483.
7
Toona sinensis and its major bioactive compound gallic acid inhibit LPS-induced inflammation in nuclear factor-κB transgenic mice as evaluated by in vivo bioluminescence imaging.香椿及其主要生物活性化合物没食子酸通过体内生物发光成像评价抑制核因子-κB 转基因小鼠脂多糖诱导的炎症。
Food Chem. 2013 Jan 15;136(2):426-34. doi: 10.1016/j.foodchem.2012.08.009. Epub 2012 Aug 17.
8
Antioxidant and immunomodulatory activities of polysaccharides from the roots of Sanguisorba officinalis.地榆根多糖的抗氧化和免疫调节活性。
Int J Biol Macromol. 2012 Dec;51(5):1057-62. doi: 10.1016/j.ijbiomac.2012.08.019. Epub 2012 Aug 27.
9
Polygoni Rhizoma inhibits inflammatory response through inactivation of nuclear factor-kappaB and mitogen activated protein kinase signaling pathways in RAW264.7 mouse macrophage cells.何首乌通过抑制 RAW264.7 小鼠巨噬细胞中核因子-κB 和丝裂原活化蛋白激酶信号通路来抑制炎症反应。
Phytother Res. 2012 Feb;26(2):239-45. doi: 10.1002/ptr.3530. Epub 2011 Jun 7.
10
The pro- and anti-inflammatory properties of the cytokine interleukin-6.细胞因子白细胞介素-6的促炎和抗炎特性。
Biochim Biophys Acta. 2011 May;1813(5):878-88. doi: 10.1016/j.bbamcr.2011.01.034. Epub 2011 Feb 4.