体外研究没食子酸联合氟化钠治疗牙本质敏感症。

In vitro study of the treatment of dentin hypersensitivity with gallic acid combined with sodium fluoride.

机构信息

Department of Stomatology, Zhangdian District Second People's Hospital, Zibo, Shandong Province, PR China.

Clinical laboratory, Shandong Healthcare Group Zibo Hospital, Zibo, Shandong Province, PR China.

出版信息

BMC Oral Health. 2024 Oct 30;24(1):1319. doi: 10.1186/s12903-024-05098-5.

DOI:10.1186/s12903-024-05098-5

PMID:39478528

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11523638/

Abstract

BACKGROUND

Dentin hypersensitivity (DH) is a common oral condition that is associated with severe dental pain. Pain relief is a key focus of the treatment of DH. The purpose of this study was to evaluate the blocking and antacid effects of gallic acid (GA) combined with sodium fluoride (NaF) on dentinal tubules in vitro.

METHODS

Ninety dentin discs from human third molars were treated with 6% citric acid for 2 min. Then, the surface morphologies of ten dentin discs were observed by scanning electron microscopy (SEM). The remaining samples were randomly divided into four groups: the NaF group, which was treated with 1000 ppm NaF; the GA group, which was treated with 4000 ppm GA; the GA + NaF group, which was treated with 1000 ppm NaF + 4000 ppm GA; and the blank group, which was treated with deionized water. The dentin permeability of each sample was measured with a water-filled system before processing and after 7 days of treatment. Dentin morphology and surface deposits were observed by SEM. Then, samples from the NaF, GA + NaF and blank groups were subjected to an acid challenge by incubation with 0.02% citric acid for 2 min. SEM and a water-filled system were used to evaluate the blocking and antacid effects of NaF and GA + NaF.

RESULTS

NaF and GA + NaF significantly decreased dentin permeability. The effect of the GA + NaF treatment was more significant. After acid challenge, both groups still exhibited decreased dentin permeability compared with the initial assessment. 2. Compared with the NaF group, the GA + NaF group had more mineral deposits on the dentin surface and dentin tubules. After acid challenge, the deposits in the GA + NaF group were still clearly visible.

CONCLUSION

The combined effect of GA and NaF on reducing dentin permeability by blocking open dentin tubules is better than that of NaF alone. After acid challenge, the GA + NaF treatment still had a better effect.

摘要

背景

牙本质敏感（DH）是一种常见的口腔疾病，与严重的牙齿疼痛有关。缓解疼痛是 DH 治疗的重点。本研究旨在评估没食子酸（GA）联合氟化钠（NaF）对体外牙本质小管的阻断和抗酸作用。

方法

用 6%柠檬酸处理 90 个人第三磨牙牙本质片 2 分钟。然后，用扫描电子显微镜（SEM）观察 10 个牙本质片的表面形态。其余样本随机分为四组：NaF 组，用 1000 ppm NaF 处理；GA 组，用 4000 ppm GA 处理；GA+NaF 组，用 1000 ppm NaF+4000 ppm GA 处理；空白组，用去离子水处理。处理前后，用充水系统测量各样本的牙本质通透性。用 SEM 观察牙本质形态和表面沉积物。然后，用 0.02%柠檬酸孵育 2 分钟，对 NaF、GA+NaF 和空白组的样本进行酸挑战。用 SEM 和充水系统评价 NaF 和 GA+NaF 的阻断和抗酸作用。