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苍耳子果实的植物化学分析：定量及对炎症反应的抑制作用

Phytochemical Analysis on Quantification and the Inhibitory Effects on Inflammatory Responses from the Fruit of Xanthii fructus.

作者信息

Yoo Sae-Rom, Seo Chang-Seob, Lee Na-Ri, Shin Hyeun-Kyoo, Jeong Soo-Jin

机构信息

K-herb Research Center, Daejeon, Republic of Korea.

KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea; Korean Medicine Life Science, University of Science and Technology, Daejeon, Republic of Korea.

出版信息

Pharmacogn Mag. 2015 Oct;11(Suppl 4):S585-91. doi: 10.4103/0973-1296.172966.

DOI:10.4103/0973-1296.172966
PMID:27013799
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4787093/
Abstract

OBJECTIVE

Xanthii fructus (Compositae) is a traditional herbal medicine used for treating headache, toothache, pruritus, empyema, and rhinitis. In this study of the quality control of X. fructus, we performed simultaneous analysis of nine marker compounds: Protocatechuic acid (1), chlorogenic acid (2), caffeic acid (3), 4,5-dicaffeoylquinic acid (4), ferulic acid (5), 3,5-dicaffeoylquinic acid (6), 1,3-dicaffeoylquinic acid (7), 1,4-dicaffeoylquinic acid (8), and 4,5-dicaffeoylquinic acid (9).

MATERIALS AND METHODS

Nine components were separated using reversed-phase SunFire™ C18 analytical column and analyzed using high-performance liquid chromatography. We examined the biological effects of the nine marker compounds by determining their anti-inflammatory activities in the murine macrophage cell line RAW 264.7.

RESULTS

Among the nine marker compounds, eight significantly inhibited lipopolysaccharide (LPS)-stimulated tumor necrosis factor-alpha (TNF-α) production. 1, 3, 5 had significant inhibitory effects on LPS-induced prostaglandin E2 (PGE2) production in RAW 264.7 cells. None of the tested marker compounds had a significant effect on interleukin-6 production in LPS-treated RAW 264.7 cells. Our data demonstrated that each marker compound from X. fructus exerts anti-inflammatory activity by targeting different inflammation-related pathways such as the TNF-α or PGE2 pathway.

CONCLUSION

Further experiments using in vitro and in vivo models are needed to identify the mechanisms responsible for the anti-inflammatory properties of each marker compound.

SUMMARY

Simultaneous analysis of nine phenylpropanoids in the Xanthii fructus was established using HPLC-PDA system.1,4-dicaffeoylquinic acid significantly inhibited LPS-stimulated TNF-a production.Protocatechuic acid, caffeic acid and ferulic acid had significant inhibitory effects on LPS-induced PGE2 production in RAW 264.7 cells.

摘要

目的

苍耳子（菊科）是一种传统草药，用于治疗头痛、牙痛、瘙痒、脓胸和鼻炎。在这项关于苍耳子质量控制的研究中，我们对9种标记化合物进行了同步分析：原儿茶酸（1）、绿原酸（2）、咖啡酸（3）、4,5-二咖啡酰奎宁酸（4）、阿魏酸（5）、3,5-二咖啡酰奎宁酸（6）、1,3-二咖啡酰奎宁酸（7）、1,4-二咖啡酰奎宁酸（8）和4,5-二咖啡酰奎宁酸（9）。

材料与方法

使用反相SunFire™ C18分析柱分离9种成分，并采用高效液相色谱法进行分析。我们通过测定9种标记化合物在小鼠巨噬细胞系RAW 264.7中的抗炎活性，来研究它们的生物学效应。

结果

在9种标记化合物中，8种显著抑制脂多糖（LPS）刺激的肿瘤坏死因子-α（TNF-α）生成。1、3、5对RAW 264.7细胞中LPS诱导的前列腺素E2（PGE2）生成有显著抑制作用。在所测试的标记化合物中，没有一种对LPS处理的RAW 264.7细胞中白细胞介素-6的生成有显著影响。我们的数据表明，苍耳子中的每种标记化合物通过靶向不同的炎症相关途径（如TNF-α或PGE2途径）发挥抗炎活性。

结论

需要使用体外和体内模型进行进一步实验，以确定每种标记化合物抗炎特性的作用机制。

总结

采用HPLC-PDA系统建立了苍耳子中9种苯丙素类化合物的同步分析方法。1,4-二咖啡酰奎宁酸显著抑制LPS刺激的TNF-α生成。原儿茶酸、咖啡酸和阿魏酸对RAW 264.7细胞中LPS诱导的PGE2生成有显著抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681c/4787093/900ff0ad302c/PM-11-585-g013.jpg
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