Wang Lianghai, Li Jing, Hou Jun, Li Man, Cui Xiaobin, Li Shugang, Yu Xiaodan, Zhang Zhiyu, Liang Weihua, Jiang Jinfang, Pang Lijuan, Chen Yunzhao, Zhao Jin, Li Feng
Department of Pathology and Key Laboratories for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang China.
Department of Immunology, Shihezi University School of Medicine, Shihezi, Xinjiang China.
Infect Agent Cancer. 2016 Apr 13;11:19. doi: 10.1186/s13027-016-0065-x. eCollection 2016.
p16(INK4A) expression has been used as a surrogate marker for human papillomavirus (HPV) infection in cervical cancer and head and neck cancer. p53 has also been reported as a feasible marker to identify HPV-positive oropharyngeal carcinoma and penile lesions. This study aimed to investigate p16(INK4A) and p53 expression levels and their correlation with HPV status and clinical parameters in Kazakh patients with esophageal squamous cell carcinoma.
Immunohistochemical expression of p16 (INK4A) and p53 were evaluated in 163 cases of esophageal squamous cell carcinoma in Kazakh patients. The presence of HPV DNA was detected by polymerase chain reaction.
p16 (INK4A) -positive expression was detected in 19.0 % of patients, and its expression was significantly correlated with a lower frequency of lymph node metastasis (p = 0.038). By contrast no significant association was found between p16 (INK4A) -positive expression and HPV status (correlation coefficient = -0.062, p = 0.499). p16 (INK4A) -positive expression did not affect the odds of tumors being HPV positive (odds ratio [OR] = 0.727 with 95 % confidence interval [CI] = 0.288-1.836). The sensitivity of p16 (INK4A) -positive expression as an HPV marker was 0.164, with a specificity of 0.788 and a positive predictive value of 0.391. p53-positive expression was present in 88.3 % of all cases. Although no significant correlation with available clinical parameters was found, a significantly inverse correlation was observed between p53 expression and HPV status (correlation coefficient = -0.186, p = 0.039). Moreover, p53-positive expression decreased the odds of tumors being HPV positive (OR = 0.292 with 95 % CI = 0.086-0.990). The sensitivity of p53-negative expression as an HPV marker was 0.179, with a specificity of 0.940 and a positive predictive value of 0.714. The overall HPV prevalence was high (45.5 %) in Kazakh patients, with no significant association between HPV positivity and available clinical parameters or combined p16 (INK4A) /p53 expression.
p16 (INK4A) -positive expression was associated with lymph node metastasis. Results indicate that p53-negative expression and not p16 (INK4A) -positive expression may be used as a marker for HPV status in ESCC; however, this finding requires further studies for validation.
p16(INK4A)表达已被用作宫颈癌和头颈癌中人乳头瘤病毒（HPV）感染的替代标志物。p53也被报道为识别HPV阳性口咽癌和阴茎病变的可行标志物。本研究旨在调查哈萨克族食管鳞状细胞癌患者中p16(INK4A)和p53的表达水平及其与HPV状态和临床参数的相关性。
对163例哈萨克族食管鳞状细胞癌患者进行p16(INK4A)和p53的免疫组化表达评估。通过聚合酶链反应检测HPV DNA的存在。
19.0%的患者检测到p16(INK4A)阳性表达，其表达与较低的淋巴结转移频率显著相关（p = 0.038）。相比之下，未发现p16(INK4A)阳性表达与HPV状态之间存在显著关联（相关系数 = -0.062，p = 0.499）。p16(INK4A)阳性表达不影响肿瘤为HPV阳性的几率（优势比[OR] = 0.727，95%置信区间[CI] = 0.288 - 1.836）。p16(INK4A)阳性表达作为HPV标志物的敏感性为0.164，特异性为0.788，阳性预测值为0.391。88.3%的病例存在p53阳性表达。虽然未发现与现有临床参数有显著相关性，但观察到p53表达与HPV状态之间存在显著负相关（相关系数 = -0.186，p = 0.039）。此外，p53阳性表达降低了肿瘤为HPV阳性的几率（OR = 0.292，95% CI = 0.086 - 0.990）。p53阴性表达作为HPV标志物的敏感性为0.179，特异性为0.940，阳性预测值为0.714。哈萨克族患者的总体HPV感染率较高（45.5%），HPV阳性与现有临床参数或联合p16(INK4A)/p53表达之间无显著关联。
p16(INK4A)阳性表达与淋巴结转移有关。结果表明，p53阴性表达而非p16(INK4A)阳性表达可能用作食管鳞状细胞癌中HPV状态的标志物；然而，这一发现需要进一步研究进行验证。
