Pastrez Paula Roberta Aguiar, Mariano Vânia Sammartino, da Costa Allini Mafra, Silva Estela Maria, Scapulatempo-Neto Cristovam, Guimarães Denise Peixoto, Fava Gilberto, Neto Said Abdala Zemi, Nunes Emily Montosa, Sichero Laura, Villa Luisa Lina, Syrjanen Kari Juhani, Longatto-Filho Adhemar
Teaching and Research Institute, Molecular Oncology Research Center, Barretos Cancer Hospital - Pio XII Foundation, Brazil.
Department of Endoscopy, Barretos Cancer Hospital - Pio XII Foundation, Brazil.
J Cancer. 2017 Apr 9;8(6):1062-1070. doi: 10.7150/jca.17080. eCollection 2017.
To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma. The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC). High-risk HPV (hr-HPV) was found in the same frequency (13.8%) in esophageal squamous cell carcinoma (ESCC) and in healthy individuals. The p53 expression was positive in 67.5% of tumor tissue, 20.0% of adjacent non-tumoral tissue and 1.8% of normal esophageal tissue. p16 was positive in 11.6% of esophageal cancer cases and 4.7% of adjacent non-tumoral tissue. p16 was undetectable among control group samples. p53 and p16 levels were not significantly associated with the HPV status. These results suggest that hr-HPV types are not associated with the development of ESCC and that p53 and p16 protein expression have no relationship with HPV infection in normal or cancerous esophagus.
为了研究人乳头瘤病毒（HPV）的流行情况，并描述食管鳞状细胞癌中HPV感染潜在分子替代标志物的表达特征。通过多重聚合酶链反应（PCR）确定患有和未患有食管癌（EC）个体中HPV的流行情况；通过免疫组织化学（IHC）评估p16和p53蛋白水平。在食管鳞状细胞癌（ESCC）和健康个体中发现高危型HPV（hr-HPV）的频率相同（13.8%）。p53在67.5%的肿瘤组织、20.0%的相邻非肿瘤组织和1.8%的正常食管组织中表达呈阳性。p16在11.6%的食管癌病例和4.7%的相邻非肿瘤组织中呈阳性。在对照组样本中未检测到p16。p53和p16水平与HPV状态无显著相关性。这些结果表明，hr-HPV类型与ESCC的发生无关，并且p53和p16蛋白表达与正常或癌性食管中的HPV感染无关。
