Huynh Nhat, Arabian Natalie, Lieu Dustin, Asatryan Liana, Davies Daryl L
Titus Family Department of Clinical Pharmacy and Pharmaceutical Economics & Policy, University of Southern California School of Pharmacy.
Titus Family Department of Clinical Pharmacy and Pharmaceutical Economics & Policy, University of Southern California School of Pharmacy;
J Vis Exp. 2016 Mar 23(109):e53770. doi: 10.3791/53770.
Prior to testing novel therapeutics in humans, short and long term preclinical (i.e., animal), repetitive pharmacological and toxicological testing is required. In most cases, the preferred route of administration is via oral delivery. At the present time, oral delivery is mostly accomplished using an oral gavage procedure, in part, because it can achieve consistent and precise dosing in the animal model. Although this method is well established it does have complications that can result in a high rate of animal attrition. To this end, the procedure introduced here describes an alternative to the oral gavage method in which the desired drug is incorporated into a tastant, orally dissolving strip (ODS) that can simply be presented to the test animal where it is then rapidly taken up with minimal manipulation of the test subject. Herein, we demonstrate that preclinical, oral drug delivery using the ODS method represents a safe, convenient, and humane alternative to oral gavage.
在对新型疗法进行人体试验之前，需要进行短期和长期的临床前（即动物）重复性药理学和毒理学测试。在大多数情况下，首选的给药途径是口服。目前，口服给药大多通过灌胃操作来完成，部分原因是它可以在动物模型中实现一致且精确的给药剂量。尽管这种方法已被广泛应用，但它确实存在一些并发症，可能导致较高的动物损耗率。为此，本文介绍的方法描述了一种灌胃法的替代方法，即将所需药物掺入一种味觉剂——口腔溶解条（ODS）中，该条可以简单地呈现给试验动物，然后在对试验对象进行最少操作的情况下迅速被其摄取。在此，我们证明使用ODS方法进行临床前口服给药是一种安全、方便且人道的灌胃替代方法。
