Bogusławska-Tryk M, Piotrowska A, Szymeczko R, Burlikowska K
Department of Animal Physiology and Morphology, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland.
J Anim Physiol Anim Nutr (Berl). 2016 Dec;100(6):1050-1057. doi: 10.1111/jpn.12476. Epub 2016 Apr 14.
The study was conducted to determine the effect of a lignocellulose supplemented diet on apparent ileal and total tract digestibility of fat and fatty acids (FA) in broiler chickens. A total of 48 21-day-old male Ross 308 chickens were divided into four treatment groups (n = 12) with six replicates per treatment. From 21 to 42 days of age, the broilers were fed experimental diets varied in the amount of lignocellulose: 0%, 0.25%, 0.5% and 1%. Total excreta were gathered during the last 3 days of the feeding trial and digesta was collected from the ileum at 42 days of the bird age. Digestibility was determined by the indicator method. The ether extract content in diet/digesta/excreta was determined by the gravimetric method, and fatty acid methyl esters were analysed by gas chromatography-mass spectrometry. Fat digestibility measured to the end of the small intestine and in the whole gastrointestinal tract in birds was high and exceeded 90% and 87% respectively. Addition of lignocellulose (1%) increased (p < 0.05) ileal fat digestibility but had no significant effect on total tract fat digestion. Absorption of total fatty acids (TFA) as well as myristic (C14:0), palmitoleic (C16:1) and α-linolenic (C18:3n-3) acids, estimated by both methods, was significantly higher in birds fed the diets supplemented with lignocellulose, especially at a dose of 1%. Total tract absorption of some dietary polyunsaturated fatty acids (PUFA) (C20:2, C20:4n-6) was significantly lower from diet supplemented with 0.5% and 0.25% lignocellulose. There was observed a decrease in apparent digestibility of fat and most examined fatty acids, when measured between terminal ileum and total gastrointestinal tract. The results suggest that lignocellulose can affect digestion and FA absorption in broilers but, as the effect of lignocellulose was not studied previously, further investigations are necessary to confirm the results of the present experiment.
本研究旨在确定添加木质纤维素的日粮对肉鸡回肠表观消化率以及脂肪和脂肪酸（FA）全肠道消化率的影响。将48只21日龄的雄性罗斯308肉鸡分为四个处理组（每组n = 12），每个处理重复六次。从21日龄到42日龄，给肉鸡饲喂木质纤维素含量不同的试验日粮：0%、0.25%、0.5%和1%。在饲养试验的最后3天收集全部排泄物，并在鸡42日龄时从回肠采集食糜。消化率采用指示剂法测定。日粮/食糜/排泄物中的乙醚提取物含量采用重量法测定，脂肪酸甲酯通过气相色谱 - 质谱联用仪进行分析。在鸡中，小肠末端和整个胃肠道的脂肪消化率较高，分别超过90%和87%。添加1%的木质纤维素可提高（p < 0.05）回肠脂肪消化率，但对全肠道脂肪消化无显著影响。通过两种方法估计，饲喂添加木质纤维素日粮的鸡对总脂肪酸（TFA）以及肉豆蔻酸（C14:0）、棕榈油酸（C16:1）和α - 亚麻酸（C18:3n - 3）的吸收显著更高，尤其是在添加量为1%时。添加0.5%和0.25%木质纤维素的日粮中，一些膳食多不饱和脂肪酸（PUFA）（C20:2、C20:4n - 6）的全肠道吸收显著降低。当在回肠末端和整个胃肠道之间进行测量时，观察到脂肪和大多数检测脂肪酸的表观消化率有所下降。结果表明，木质纤维素可影响肉鸡的消化和脂肪酸吸收，但由于此前未对木质纤维素的作用进行研究，因此需要进一步研究以证实本实验结果。
