Mélo S K M, Diniz A I A, de Lira V L, de Oliveira Muniz S K, da Silva G R, Manso H E C da C C, Manso Filho H C
Equine Research Center & Laboratory of Applied Molecular Biology for Animal Production, Department of Animal Sciences, Rural Federal University of Pernambuco, Recife, Brazil.
J Anim Physiol Anim Nutr (Berl). 2016 Oct;100(5):852-9. doi: 10.1111/jpn.12456. Epub 2016 Apr 14.
Oxidative stress has been correlated with pathologies that impair the performance of athlete horses. The aim of this study was to assess the effects of supplementation with a mixture of polyunsaturated oil and vitamin E on the antioxidant and haematological biomarkers of horses. Horses under maintenance care (n = 6) and horses in training (n = 10) received 100 and 300 ml of the oil mixture respectively. Supplementation was provided for a period of 8 weeks, together with isocaloric inclusion. Blood samples were collected at three time periods (pretest, after 4 weeks and after 8 weeks) to analyse the following: the red blood cell count (RBCc); haemoglobin (Hb); haematocrit (HT); leucocytes; lymphocytes; platelets; the mean corpuscular volume (MCV); the mean corpuscular haemoglobin concentration (MCHC); the standard deviation of the red blood cell distribution width (RDW-SD); the coefficient of variation of the red blood cell distribution width (RDW-CV); glutathione peroxidase (GPx); superoxide dismutase (SOD); uric acid (UrAc); total plasma proteins (TPP); and creatine kinase (CK). After the 8 weeks of supplementation, animals under maintenance care exhibited significant increases in SOD, UrAc, the white blood cell count (WBCc), the RDW-SD and the RDW-CV (p < 0.05). The animals in training exhibited increases in GPx, SOD and UrAc (p < 0.05). In conclusion, supplementation with polyunsaturated oil and vitamin E increases blood antioxidants among animals under maintenance and in training, with different trends, while contributing to the fight against oxidative stress in each group analysed.
氧化应激与损害竞技马匹表现的病理状况相关。本研究的目的是评估补充多不饱和油和维生素E的混合物对马的抗氧化和血液学生物标志物的影响。处于维持护理状态的马（n = 6）和训练中的马（n = 10）分别接受100毫升和300毫升的油混合物。在8周的时间内进行补充，并提供等热量的食物。在三个时间段（预测试、4周后和8周后）采集血样，以分析以下指标：红细胞计数（RBCc）；血红蛋白（Hb）；血细胞比容（HT）；白细胞；淋巴细胞；血小板；平均红细胞体积（MCV）；平均红细胞血红蛋白浓度（MCHC）；红细胞分布宽度标准差（RDW-SD）；红细胞分布宽度变异系数（RDW-CV）；谷胱甘肽过氧化物酶（GPx）；超氧化物歧化酶（SOD）；尿酸（UrAc）；总血浆蛋白（TPP）；以及肌酸激酶（CK）。补充8周后，处于维持护理状态的动物的SOD、UrAc、白细胞计数（WBCc）、RDW-SD和RDW-CV显著增加（p < 0.05）。训练中的动物的GPx、SOD和UrAc增加（p < 0.05）。总之，补充多不饱和油和维生素E可使处于维持护理状态和训练中的动物的血液抗氧化剂增加，且趋势不同，同时有助于在所分析的每组中对抗氧化应激。
