Yolbas Servet, Kara Murat, Yilmaz Musa, Aydin Suleyman, Koca Suleyman Serdar
Department of Rheumatology, Faculty of Medicine, Firat University, 23119, Elazig, Turkey.
Department of Medical Genetics, Faculty of Medicine, Mugla Sitki Kocman University, Mugla, Turkey.
Clin Rheumatol. 2016 Jun;35(6):1535-40. doi: 10.1007/s10067-016-3266-1. Epub 2016 Apr 15.
Adropin, a secreted protein, is encoded by the energy homeostasis associated (ENHO) gene. It has been implicated in the several physiological and pathological processes such as angiogenesis and apoptosis. Therefore, the aim of present study was to investigate serum adropin levels and ENHO gene expressions in systemic sclerosis (SSc) characterized by vasculopathy, inflammation, and progressive fibrosis of the skin and internal organs. The study includes 27 patients with SSc, 39 patients with Behçet's disease (BD), and 20 healthy controls (HC). Serum adropin levels and ENHO gene expressions by peripheral blood mononuclear cells were analyzed by ELISA method and by real-time PCR, respectively. The serum adropin levels were higher in the SSc and BD groups than in the HC group (p = 0.023 and p < 0.001, respectively). However, there were no significant differences among the groups in terms of ENHO gene expressions (p ANOVA = 0.149). There was no significant difference between the limited and diffuse cutaneous subtypes of SSc in terms of serum adropin level and ENHO gene expression. Moreover, serum adropin level and ENHO gene expression were not associated with the disease activity and severity indexes. ENHO gene expression was correlated with the triglyceride levels in the BD group (r = -0.426, p = 0.027). The augmented serum adropin levels may be expected in the chronic inflammatory disease and seem not to be characteristic of only SSc. However, further studies are needed to explain the precise role of adropin in SSc.
内脂素是一种分泌蛋白，由能量稳态相关（ENHO）基因编码。它参与了诸如血管生成和细胞凋亡等多种生理和病理过程。因此，本研究的目的是调查系统性硬化症（SSc）患者的血清内脂素水平和ENHO基因表达，SSc的特征为血管病变、炎症以及皮肤和内脏器官的进行性纤维化。该研究纳入了27例SSc患者、39例白塞病（BD）患者和20名健康对照者（HC）。分别采用酶联免疫吸附测定法（ELISA）和实时聚合酶链反应（real-time PCR）分析外周血单个核细胞的血清内脂素水平和ENHO基因表达。SSc组和BD组的血清内脂素水平高于HC组（分别为p = 0.023和p < 0.001）。然而，各组之间在ENHO基因表达方面无显著差异（p方差分析 = 0.149）。SSc的局限性皮肤亚型和弥漫性皮肤亚型在血清内脂素水平和ENHO基因表达方面无显著差异。此外，血清内脂素水平和ENHO基因表达与疾病活动度和严重程度指标无关。在BD组中，ENHO基因表达与甘油三酯水平相关（r = -0.426，p = 0.027）。慢性炎症性疾病患者的血清内脂素水平可能会升高，而且似乎并非SSc所特有。然而，需要进一步研究来解释内脂素在SSc中的精确作用。
