Nakano M M, Butsuya I, Ogawara H
Department of Biochemistry, Meiji College of Pharmacy, Tokyo, Japan.
J Antibiot (Tokyo). 1989 Mar;42(3):423-30. doi: 10.7164/antibiotics.42.423.
The previously cloned kanamycin resistance gene (kmr) from Streptomyces kanamyceticus ISP5500 was shown to modify the 30S ribosomal subunit in a subunit exchange experiment. The kmr gene, which was normally repressed in S. kanamyceticus, appeared to be induced under growth conditions which activated kanamycin biosynthesis. S1 mapping analysis revealed that the expression of the kmr gene was regulated at the transcriptional level. Acetylation of kanamycin is another resistance mechanism in the kanamycin producer. However, unlike kmr-mediated resistance, the enzyme which catalyzed acetylation was not regulated coordinately with kanamycin biosynthesis.
先前从卡那霉素链霉菌ISP5500克隆的卡那霉素抗性基因（kmr）在亚基交换实验中显示可修饰30S核糖体亚基。kmr基因在卡那霉素链霉菌中通常受到抑制，但在激活卡那霉素生物合成的生长条件下似乎会被诱导。S1图谱分析表明，kmr基因的表达在转录水平受到调控。卡那霉素的乙酰化是卡那霉素产生菌中的另一种抗性机制。然而，与kmr介导的抗性不同，催化乙酰化的酶与卡那霉素生物合成没有协同调控。
