Nakano M M, Mashiko H, Ogawara H
J Bacteriol. 1984 Jan;157(1):79-83. doi: 10.1128/jb.157.1.79-83.1984.
A kanamycin-producing strain, Streptomyces kanamyceticus ISP5500, is resistant to kanamycin. A kanamycin resistance determinant was cloned from S. kanamyceticus into Streptomyces lividans 1326, using the plasmid vector pIJ702. The resulting plasmid, pMCP5, could also transform Streptomyces lavendulae S985 and Streptomyces parvulus 2283 to kanamycin resistance. Transformants carrying pMCP5 were markedly more resistant than S. kanamyceticus to the aminoglycoside antibiotics sisomicin, tobramycin, amikacin, and gentamicin. Studies in vitro polyphenylalanine synthesis showed that strains carrying pMCP5 contained kanamycin-resistant ribosomes. However, growing S. kanamyceticus contained kanamycin-sensitive ribosomes. Ribosomes from S. kanamyceticus grown under kanamycin-producing conditions were kanamycin resistant.
产卡那霉素的菌株——卡那霉素链霉菌ISP5500对卡那霉素具有抗性。利用质粒载体pIJ702，从卡那霉素链霉菌中克隆出卡那霉素抗性决定簇，并导入变铅青链霉菌1326中。所得质粒pMCP5也能将薰衣草链霉菌S985和微小链霉菌2283转化为对卡那霉素具有抗性。携带pMCP5的转化体比卡那霉素链霉菌对氨基糖苷类抗生素西索米星、妥布霉素、阿米卡星和庆大霉素的抗性明显更强。体外聚苯丙氨酸合成研究表明，携带pMCP5的菌株含有对卡那霉素具有抗性的核糖体。然而，正在生长的卡那霉素链霉菌含有对卡那霉素敏感的核糖体。在产生卡那霉素的条件下生长的卡那霉素链霉菌的核糖体对卡那霉素具有抗性。
