来自产卡那霉素的卡那链霉菌IFO 13414的卡那霉素乙酰转移酶基因。

Kanamycin acetyltransferase gene from kanamycin-producing Streptomyces kanamyceticus IFO 13414.

作者信息

Joe Y A, Goo Y M

机构信息

College of Pharmacy, Seoul National University, Korea.

出版信息

Arch Pharm Res. 1998 Aug;21(4):470-4. doi: 10.1007/BF02974645.

DOI:10.1007/BF02974645

PMID:9875478

Abstract

A kanamycin producer, Streptomyces kanamyceticus IFO 13414 is highly resistant to kanamycin. Cloning of the kanamycin resistance genes in S. lividans 1326 with pIJ702 gave several kanamycin resistant transformants. Two transformants, S. lividans SNUS 90041 and S. lividans SNUS 91051 showed similar resistance patterns to various aminoglycoside antibiotics. Gene mapping experiments revealed that plasmids pSJ5030 and pSJ2131 isolated from the transformants have common resistant gene fragments. Subcloning of pSJ5030 gave a 1.8 Kb gene fragment which showed resistance to kanamycin. Cell free extracts of S. lividans SNUS 90041, S. lividans SNUS 91051 and subclone a S. lividans SNUS 91064 showed kanamycin acetyltransferase activity. The detailed gene map is included.

摘要

卡那霉素产生菌——卡那霉素链霉菌IFO 13414对卡那霉素具有高度抗性。用pIJ702将卡那霉素抗性基因克隆到变铅青链霉菌1326中，获得了几个卡那霉素抗性转化子。两个转化子，变铅青链霉菌SNUS 90041和变铅青链霉菌SNUS 91051对各种氨基糖苷类抗生素表现出相似的抗性模式。基因定位实验表明，从转化子中分离出的质粒pSJ5030和pSJ2131具有共同的抗性基因片段。pSJ5030的亚克隆得到了一个对卡那霉素有抗性的1.8 Kb基因片段。变铅青链霉菌SNUS 90041、变铅青链霉菌SNUS 91051和亚克隆体变铅青链霉菌SNUS 91064的无细胞提取物显示出卡那霉素乙酰转移酶活性。文中包含详细的基因图谱。