Palombo Francesca, Madami Marco, Fioretto Daniele, Nallala Jayakrupakar, Barr Hugh, David Antoine, Stone Nick
University of Exeter, School of Physics, Exeter, EX4 4QL, UK.
University of Perugia, Department of Physics and Geology, Perugia, I-06100, Italy.
J Biophotonics. 2016 Jul;9(7):694-700. doi: 10.1002/jbio.201600038. Epub 2016 Apr 18.
Barrett's oesophagus is a condition characterized by a change in the lining of the oesophagus that markedly increases the risk of adenocarcinoma. We demonstrate the first site-matched application of Brillouin microscopy, Raman microscopy and FTIR micro-spectroscopic imaging to ex-vivo epithelial tissue - Barrett's oesophagus. The mechanical and chemical characters of the epithelium were assessed in histological sections from a patient subjected to endoscopic oesophageal biopsy. Previous studies have shown that both these properties change within the oesophageal wall, owing to the presence of distinct cellular and extracellular constituents which are putatively affected by oesophageal cancer. Brillouin microscopy enables maps of elasticity of the epithelium to be obtained, whilst Raman and FTIR imaging provide 'chemical images' without the need for labelling or staining. This site-matched approach provides a valuable platform for investigating the structure, biomechanics and composition of complex heterogeneous systems. A combined Brillouin-Raman device has potential for in-vivo diagnosis of pathology. First application of site-matched micro Brillouin, Raman and FTIR spectroscopic imaging to epithelial tissue in Barrett's oesophagus.
巴雷特食管是一种以食管内膜变化为特征的疾病，这种变化会显著增加腺癌的发病风险。我们展示了布里渊显微镜、拉曼显微镜和傅里叶变换红外光谱显微镜成像首次在离体上皮组织——巴雷特食管上的位点匹配应用。通过对一名接受内镜食管活检患者的组织切片进行评估，来分析上皮组织的机械和化学特性。先前的研究表明，由于食管壁内存在不同的细胞和细胞外成分，且这些成分可能受食管癌影响，食管壁内的这两种特性都会发生变化。布里渊显微镜能够获取上皮组织弹性图谱，而拉曼成像和傅里叶变换红外成像则无需标记或染色就能提供“化学图像”。这种位点匹配方法为研究复杂异质系统的结构、生物力学和组成提供了一个有价值的平台。布里渊 - 拉曼组合设备具有用于病理学活体诊断的潜力。首次将位点匹配的显微布里渊、拉曼和傅里叶变换红外光谱成像应用于巴雷特食管的上皮组织。
