Ryu Seungmi, Martino Nicola, Kwok Sheldon J J, Bernstein Liane, Yun Seok-Hyun
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 65 Landsdowne St., Cambridge, MA 02139, USA.
National Center for Advancing Translational Sciences, National Institute of Health, Rockville, MD 20850, USA.
Biomed Opt Express. 2021 Feb 17;12(3):1437-1448. doi: 10.1364/BOE.414474. eCollection 2021 Mar 1.
Brillouin light scattering offers a unique label-free approach to measure biomechanical properties non-invasively. While this technique is used in biomechanical analysis of cells and tissues, its potential for visualizing structural features of tissues based on the biomechanical contrast has not been much exploited. Here, we present high-resolution Brillouin microscopy images of four basic tissue types: muscular, connective, epithelial, and nervous tissues. The Brillouin contrast distinguishes between muscle fiber cells and endomysium in skeletal muscle and reveals chondrocytes along with spatially varying stiffness of the extracellular matrix in articular cartilage. The hydration-sensitive contrast can visualize the stratum corneum, epidermis, and dermis in the skin epithelium. In brain tissues, the Brillouin images show the mechanical heterogeneity across the cortex and deeper regions. This work demonstrates the versatility of using the Brillouin shift as histological contrast for examining intact tissue substructures via longitudinal modulus without the need for laborious tissue processing steps.
布里渊光散射提供了一种独特的无标记方法，可用于非侵入性地测量生物力学特性。虽然该技术已用于细胞和组织的生物力学分析，但其基于生物力学对比度可视化组织结构特征的潜力尚未得到充分利用。在此，我们展示了四种基本组织类型（肌肉组织、结缔组织、上皮组织和神经组织）的高分辨率布里渊显微镜图像。布里渊对比度区分了骨骼肌中的肌纤维细胞和肌内膜，并揭示了关节软骨中的软骨细胞以及细胞外基质在空间上变化的硬度。对水合作用敏感的对比度可以可视化皮肤上皮中的角质层、表皮和真皮。在脑组织中，布里渊图像显示了整个皮质和更深区域的力学异质性。这项工作证明了使用布里渊频移作为组织学对比度，通过纵向模量检查完整组织亚结构的多功能性，而无需繁琐的组织处理步骤。
