Yumerefendi Hayretin, Lerner Andrew Michael, Zimmerman Seth Parker, Hahn Klaus, Bear James E, Strahl Brian D, Kuhlman Brian
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA.
Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA.
Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.
我们设计了一种光激活系统，通过将核输出信号嵌入光敏色素1的LOV2结构域，实现蛋白质从细胞核的快速可逆输出。将染色质修饰因子Bre1与光开关融合，我们实现了对酵母中组蛋白H2B单泛素化的光依赖性控制，揭示了泛素标记的快速周转。此外，这种诱导系统使我们能够动态监测依赖于H2B泛素化的表观遗传修饰状态。
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