Gene expression and mRNA Metabolism Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain.
Gene expression and mRNA Metabolism Laboratory, Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Científicas (CSIC), Valencia, Spain.
EMBO Rep. 2018 Nov;19(11). doi: 10.15252/embr.201845992. Epub 2018 Sep 24.
Monoubiquitination of histone H2B (to H2Bub1) is required for downstream events including histone H3 methylation, transcription, and mRNA export. The mechanisms and players regulating these events have not yet been completely delineated. Here, we show that the conserved Ran-binding protein Mog1 is required to sustain normal levels of H2Bub1 and H3K4me3 in Mog1 is needed for gene body recruitment of Rad6, Bre1, and Rtf1 that are involved in H2B ubiquitination and genetically interacts with these factors. We provide evidence that the absence of impacts on cellular processes such as transcription, DNA replication, and mRNA export, which are linked to H2Bub1. Importantly, the mRNA export defect in Δ strains is exacerbated by the absence of factors that decrease H2Bub1 levels. Consistent with a role in sustaining H2Bub and H3K4me3 levels, Mog1 co-precipitates with components that participate in these modifications such as Bre1, Rtf1, and the COMPASS-associated factors Shg1 and Sdc1. These results reveal a novel role for Mog1 in H2B ubiquitination, transcription, and mRNA biogenesis.
组蛋白 H2B 的单泛素化（至 H2Bub1）是下游事件所必需的，包括组蛋白 H3 甲基化、转录和 mRNA 输出。调节这些事件的机制和参与者尚未完全描述。在这里，我们表明，保守的 Ran 结合蛋白 Mog1 对于维持正常水平的 H2Bub1 和 H3K4me3 是必需的，Mog1 对于 Rad6、Bre1 和 Rtf1 的基因体募集是必需的，这些因子参与 H2B 泛素化，并与这些因子在遗传上相互作用。我们提供的证据表明，缺失 Mog1 会影响转录、DNA 复制和与 H2Bub1 相关的 mRNA 输出等细胞过程。重要的是，在缺失降低 H2Bub1 水平的因子的情况下，Δ 菌株中的 mRNA 输出缺陷会加剧。与维持 H2Bub 和 H3K4me3 水平的作用一致，Mog1 与参与这些修饰的组分（如 Bre1、Rtf1 和与 COMPASS 相关的因子 Shg1 和 Sdc1）共沉淀。这些结果揭示了 Mog1 在 H2B 泛素化、转录和 mRNA 生物发生中的新作用。
