CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Bioresour Technol. 2016 Jul;212:144-150. doi: 10.1016/j.biortech.2016.04.027. Epub 2016 Apr 8.
In this work, oil mill wastewater (OMW), a residue generated during olive oil extraction, was evaluated as an inducer of rhamnolipid production. Using a medium containing as sole ingredients corn steep liquor (10%, v/v), sugarcane molasses (10%, w/v) and OMW (25%, v/v), Pseudomonas aeruginosa #112 produced 4.5 and 5.1g of rhamnolipid per liter in flasks and reactor, respectively, with critical micelle concentrations as low as 13mg/l. Furthermore, in the medium supplemented with OMW, a higher proportion of more hydrophobic rhamnolipid congeners was observed comparing with the same medium without OMW. OMW is a hazardous waste which disposal represents a serious environmental problem; therefore, its valorization as a substrate for the production of added-value compounds such as rhamnolipids is of great interest. This is the first report of rhamnolipid production using a mixture of these three agro-industrial by-products, which can be useful for the sustainable production of rhamnolipids.
在这项工作中，我们评估了橄榄油提取过程中产生的油厂废水（OMW）作为鼠李糖脂生产的诱导剂。使用含有玉米浆（10%，v/v）、甘蔗糖蜜（10%，w/v）和 OMW（25%，v/v）的培养基，铜绿假单胞菌#112 在摇瓶和反应器中分别产生了 4.5 和 5.1g/L 的鼠李糖脂，临界胶束浓度低至 13mg/L。此外，在补充 OMW 的培养基中，与不含 OMW 的相同培养基相比，观察到更多疏水性鼠李糖脂同系物的比例更高。OMW 是一种危险废物，其处理是一个严重的环境问题；因此，将其作为生产附加值化合物（如鼠李糖脂）的底物具有重要意义。这是首次使用这三种农业工业副产品混合物生产鼠李糖脂的报告，这对于可持续生产鼠李糖脂具有重要意义。
