Masson-Meyers Daniela Santos, Bumah Violet Vakunseh, Enwemeka Chukuka Samuel
College of Health Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI. USA.
College of Health Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI. USA.
J Photochem Photobiol B. 2016 Jul;160:53-60. doi: 10.1016/j.jphotobiol.2016.04.007. Epub 2016 Apr 9.
Irradiation with red or near infrared light promotes tissue repair, while treatment with blue light is known to be antimicrobial. Consequently, it is thought that infected wounds could benefit more from combined blue and red/infrared light therapy; but there is a concern that blue light may slow healing. We investigated the effect of blue 470nm light on wound healing, in terms of wound closure, total protein and collagen synthesis, growth factor and cytokines expression, in an in vitro scratch wound model. Human dermal fibroblasts were cultured for 48h until confluent. Then a linear scratch wound was created and irradiated with 3, 5, 10 or 55J/cm(2). Control plates were not irradiated. Following 24h of incubation, cells were fixed and stained for migration and fluorescence analyses and the supernatant collected for quantification of total protein, hydroxyproline, bFGF, IL-6 and IL-10. The results showed that wound closure was similar for groups treated with 3, 5 and 10J/cm(2), with a slight improvement with the 5J/cm(2) dose, and slower closure with 55J/cm(2) p<0.001). Total protein concentration increased after irradiation with 3, 5 and 10J/cm(2), reaching statistical significance at 5J/cm(2) compared to control (p<0.0001). However, hydroxyproline levels did not differ between groups. Similarly, bFGF and IL-10 concentrations did not differ between groups, but IL-6 concentration decreased progressively as fluence increased (p<0.0001). Fluorescence analysis showed viable cells regardless of irradiation fluence. We conclude that irradiation with blue light at low fluence does not impair in vitro wound healing. The significant decrease in IL-6 suggests that 470nm light is anti-inflammatory.
红光或近红外光照射可促进组织修复，而蓝光治疗具有抗菌作用。因此，人们认为感染伤口可能从蓝光与红光/红外光联合治疗中获益更多；但有人担心蓝光可能会延缓伤口愈合。我们在体外划痕伤口模型中，研究了470nm蓝光对伤口愈合的影响，包括伤口闭合、总蛋白和胶原蛋白合成、生长因子及细胞因子表达。将人真皮成纤维细胞培养48小时直至汇合。然后制造线性划痕伤口，并用3、5、10或55J/cm²进行照射。对照平板不进行照射。孵育24小时后，固定细胞并进行染色以进行迁移和荧光分析，收集上清液以定量总蛋白、羟脯氨酸、碱性成纤维细胞生长因子（bFGF）、白细胞介素-6（IL-6）和白细胞介素-10（IL-10）。结果显示，接受3、5和10J/cm²照射的组伤口闭合情况相似，5J/cm²剂量组有轻微改善，而55J/cm²剂量组伤口闭合较慢（p<0.001）。照射3、5和10J/cm²后总蛋白浓度增加，与对照组相比，5J/cm²时达到统计学显著差异（p<0.0001）。然而，各实验组羟脯氨酸水平无差异。同样，各实验组bFGF和IL-10浓度无差异，但IL-6浓度随辐照剂量增加而逐渐降低（p<0.0001）。荧光分析显示，无论辐照剂量如何，均有活细胞。我们得出结论，低剂量蓝光照射不会损害体外伤口愈合。IL-6的显著降低表明470nm光具有抗炎作用。
