Zhang Shu, Tian Xueli, Yin Jun, Liu Yu, Dong Zhanmin, Sun Jia-Lin, Ma Wanyun
College of Science, Huazhong Agricultural University, 430070, Wuhan, China.
State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, 100084, Beijing, China.
Sci Rep. 2016 Apr 20;6:24503. doi: 10.1038/srep24503.
Silver nanostructured films suitable for use as surface-enhanced Raman scattering (SERS) substrates are prepared in just 2 hours by the solid-state ionics method. By changing the intensity of the external direct current, we can readily control the surface morphology and growth rate of the silver nanostructured films. A detailed investigation of the surface enhancement of the silver nanostructured films using Rhodamine 6G (R6G) as a molecular probe revealed that the enhancement factor of the films was up to 10(11). We used the silver nanostructured films as substrates in SERS detection of human red blood cells (RBCs). The SERS spectra of RBCs on the silver nanostructured film could be clearly detected at a laser power of just 0.05 mW. Comparison of the SERS spectra of RBCs obtained from younger and older donors showed that the SERS spectra depended on donor age. A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors. This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people. Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.
通过固态离子法，仅需2小时就能制备出适用于表面增强拉曼散射（SERS）基底的银纳米结构薄膜。通过改变外部直流电的强度，我们可以轻松控制银纳米结构薄膜的表面形态和生长速率。以罗丹明6G（R6G）作为分子探针，对银纳米结构薄膜的表面增强进行了详细研究，结果表明该薄膜的增强因子高达10(11)。我们将银纳米结构薄膜用作基底，用于人体红细胞（RBC）的SERS检测。在仅0.05 mW的激光功率下，就能清晰检测到银纳米结构薄膜上红细胞的SERS光谱。对来自年轻和年长捐赠者的红细胞SERS光谱进行比较后发现，SERS光谱取决于捐赠者的年龄。与年轻捐赠者相比，年长捐赠者红细胞中更大比例的血红蛋白处于脱氧状态。这意味着老年人的血红蛋白携氧能力低于年轻人。总体而言，所制备的银基底在生物医学SERS光谱检测中显示出应用前景。
