Lee Jin-Ho, Lee Taek, Choi Jeong-Woo
Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea.
Institute of Integrated Biotechnology, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea.
Nanomaterials (Basel). 2016 Nov 28;6(12):224. doi: 10.3390/nano6120224.
In tissue engineering and regenerative medicine, monitoring the status of stem cell differentiation is crucial to verify therapeutic efficacy and optimize treatment procedures. However, traditional methods, such as cell staining and sorting, are labor-intensive and may damage the cells. Therefore, the development of noninvasive methods to monitor the differentiation status in situ is highly desirable and can be of great benefit to stem cell-based therapies. Toward this end, nanotechnology has been applied to develop highly-sensitive biosensors to noninvasively monitor the neural differentiation of stem cells. Herein, this article reviews the development of noninvasive nano-biosensor systems to monitor the neural differentiation of stem cells, mainly focusing on optical (plasmonic) and eletrochemical methods. The findings in this review suggest that novel nano-biosensors capable of monitoring stem cell differentiation are a promising type of technology that can accelerate the development of stem cell therapies, including regenerative medicine.
在组织工程和再生医学中，监测干细胞分化状态对于验证治疗效果和优化治疗程序至关重要。然而，传统方法，如细胞染色和分选，劳动强度大且可能损害细胞。因此，开发用于原位监测分化状态的非侵入性方法非常必要，这对于基于干细胞的治疗可能大有裨益。为此，纳米技术已被用于开发高灵敏度生物传感器，以非侵入性地监测干细胞的神经分化。本文综述了用于监测干细胞神经分化的非侵入性纳米生物传感器系统的发展，主要侧重于光学（等离子体）和电化学方法。本综述中的研究结果表明，能够监测干细胞分化的新型纳米生物传感器是一种有前途的技术类型，可以加速包括再生医学在内的干细胞治疗的发展。
