通过Sysmex XN-1000检测凋亡淋巴细胞作为单核细胞增多症综合征的诊断标志物
Detection of Apoptotic Lymphocytes Through Sysmex XN-1000 As a Diagnostic Marker for Mononucleosis Syndrome.
作者信息
Sale Silvia, Carone Addolorata Emanuela, Fumi Maurizio, Pancione Ylenia, Rocco Vincenzo
机构信息
Clinical Pathology Laboratory, A.O.R.N. "G. Rummo,", Benevento, Italy.
出版信息
J Clin Lab Anal. 2016 Sep;30(5):779-93. doi: 10.1002/jcla.21938. Epub 2016 Apr 19.
BACKGROUND
The infectious mononucleosis (IM) includes clue elements, apoptotic and atypical lymphocytes. In IM, the evaluation of dot plot provided by Sysmex XN-1000 analyzer revealed a stretched lymphocytic cluster, white cell differential channel (WDF), on cytogram.
METHODS
In this study, we analyzed 698 samples that include 39 IM, 76 chronic lymphoproliferative disorders, 25 nonclonal lymphocytosis, and 40 healthy donors. Five hundred eighteen samples with other diseases or interference were evaluated. The algorithm was validated on 40,000 files that were received from internal database of Sysmex-Dasit.
RESULTS
The analysis of flow cytometry standard (FCS) files in WDF channel and presumed apoptotic lymphocytes counts on side scatter/forward scatter (SSC/FSC) and SSC/SFL (where SFL is side fluorescence) dot plot revealed excellent correlation among apoptotic cells on peripheral blood smear (R(2) = 0.79 and 0.75). There was a variation of positional parameters in lymphocyte clusters WX, WY, and WZ. If WX-SSC > 500 and WY-SFL > 1,000 and WZ-FSC > 700, specificity equals to 99% and sensitivity equals to 100%. If nucleated red blood cell (NRBC) <0.03 × 10(3) /μl, specificity equals to 100%. In received files, positives were 1% adding the simultaneous presence of a percentage of events in the two gates relating to the apoptotic lymphocytes of 1.2% of WBC.
CONCLUSION
On Sysmex XN-1000, dot-plot observation allowed immediate detection of IM. Meanwhile, an algorithm based on the parameters on these plots can be calculated with excellent performance.
背景
传染性单核细胞增多症（IM）包括线索成分、凋亡和非典型淋巴细胞。在IM中，Sysmex XN - 1000分析仪提供的点图评估显示，细胞图上白细胞分类通道（WDF）出现淋巴细胞群拉长。
方法
在本研究中，我们分析了698份样本，包括39例IM、76例慢性淋巴细胞增殖性疾病、25例非克隆性淋巴细胞增多症和40例健康供者。对518份患有其他疾病或存在干扰的样本进行了评估。该算法在从Sysmex - Dasit内部数据库接收的40000份文件上进行了验证。
结果
对WDF通道中的流式细胞术标准（FCS）文件以及侧向散射/前向散射（SSC/FSC）和SSC/侧向荧光（SFL，其中SFL为侧向荧光）点图上推测的凋亡淋巴细胞计数进行分析，结果显示外周血涂片上的凋亡细胞之间具有良好的相关性（R² = 0.79和0.75）。淋巴细胞群WX、WY和WZ的位置参数存在差异。如果WX - SSC > 500且WY - SFL > 1000且WZ - FSC > 700，则特异性等于99%，敏感性等于100%。如果有核红细胞（NRBC）< 0.03×10³/μl，则特异性等于100%。在接收的文件中，同时存在与1.2%白细胞凋亡淋巴细胞相关的两个门中一定比例的事件时，阳性率为1%。
结论
在Sysmex XN - 1000上，通过点图观察可立即检测出IM。同时，基于这些图上参数的算法计算性能优异。
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