马尔尼菲篮状菌对希森美康 XN-9000 血液分析仪全血细胞计数的影响。

Effects of Talaromyces marneffei on Complete Blood Count by a Sysmex XN-9000 Analyzer.

出版信息

Clin Lab. 2023 Jun 1;69(6). doi: 10.7754/Clin.Lab.2022.220928.

DOI:10.7754/Clin.Lab.2022.220928

Abstract

BACKGROUND

Talaromyces marneffei (T. marneffei) infection detected in the peripheral blood smears has been described by several reports. We studied the effects of T. marneffei in peripheral blood samples on complete blood count (CBC) using a Sysmex XN-9000 analyzer.

METHODS

In a simulated T. marneffei infection model, blood samples with and without infectious diseases were selected, with high, medium, and low levels of white blood cell (WBC) and platelet (PLT) count, respectively. All samples were detected immediately and after a warm bath of 37℃ for 2 hours.

RESULTS

WBC count of all samples was significantly increased by T. marneffei from a certain concentration and higher. For all samples, the effect of T. marneffei on WBC count after warm bath was significantly reduced compared to that on immediate WBC count from 4 - 6 x 109/L T. Marneffei and higher (p < 0.05). The presence of T. marneffei in all blood samples did not affect the results of PLT count. For all samples, the obvious effects of T. marneffei on WBC differential (WDF) and white cell nucleated red blood cell (WNR) scatter plots were from 4 - 6 x 109 T Marneffei and higher.

CONCLUSIONS

As a kind of intracellular yeast, T. marneffei may affect WBC count, NRBC count, and WBC differential count of peripheral blood samples when the yeast concentration is (4 - 6) x 109 T Marneffei and higher. Moreover, the unique scatter plot cloud on WDF and WNR scatter plots caused by T. marneffei, may become an important clue pointing toward T. marneffei in peripheral blood.

摘要

背景

已有多篇报道描述了在外周血涂片上检测到的马尔尼菲篮状菌（Talaromyces marneffei，T. marneffei）感染。我们使用希森美康 XN-9000 分析仪研究了马尔尼菲篮状菌对全血细胞计数（CBC）的外周血样本的影响。

方法

在模拟的马尔尼菲篮状菌感染模型中，选择了有和无传染病的血液样本，白细胞（WBC）和血小板（PLT）计数分别为高、中、低水平。所有样本均立即检测，并在 37℃温浴 2 小时后进行检测。

结果

从一定浓度及更高浓度开始，所有样本的 WBC 计数均被 T. marneffei 显著增加。对于所有样本，温浴后 T. marneffei 对 WBC 计数的影响与立即检测相比明显降低，从 4-6×109/L T. Marneffei 及更高浓度开始（p<0.05）。所有血液样本中存在 T. marneffei 并不影响 PLT 计数的结果。对于所有样本，T. marneffei 对 WBC 差异（WDF）和白细胞有核红细胞（WNR）散点图的明显影响从 4-6×109 T Marneffei 及更高浓度开始。

结论

作为一种细胞内酵母，当酵母浓度为（4-6）×109 T Marneffei 及更高时，T. marneffei 可能会影响外周血样本的 WBC 计数、NRBC 计数和 WBC 差异计数。此外，T. marneffei 在 WDF 和 WNR 散点图上产生的独特散点云图，可能成为外周血中指向马尔尼菲篮状菌的重要线索。