Lv Dong-Wen, Zhu Geng-Rui, Zhu Dong, Bian Yan-Wei, Liang Xiao-Na, Cheng Zhi-Wei, Deng Xiong, Yan Yue-Ming
College of Life Science, Capital Normal University, 100048 Beijing, China; VCU Philips Institute for Oral Health Research, Virginia Commonwealth University, School of Dentistry, Department of Oral and Craniofacial Molecular Biology, 23298 Richmond, VA, USA.
College of Life Science, Capital Normal University, 100048 Beijing, China.
J Proteomics. 2016 Jun 30;143:93-105. doi: 10.1016/j.jprot.2016.04.013. Epub 2016 Apr 16.
Salinity is a major abiotic stress factor affecting crops production and productivity. Triticum monococcum is closely related to Triticum urartu (A(U)A(U)), which is used as a model plant of wheat A genome study. Here, salt stress induced dynamic proteome and phosphoproteome profiling was focused. The T. monococcum seedlings were initially treated with different concentrations of NaCl ranging from 80 to 320mM for 48h followed by a recovery process for 48h prior to proteomic and phosphoproteomic analysis. As a result, a total of 81 spots corresponding to salt stress and recovery were identified by MALDI-TOF/TOF-MS from 2-DE gels. These proteins were mainly involved in regulatory, stress defense, protein folding/assembly/degradation, photosynthesis, carbohydrate metabolism, energy production and transportation, protein metabolism, and cell structure. Pro-Q Diamond staining was used to detect the phosphoproteins. Finally, 20 spots with different phosphorylation levels during salt treatment or recovery compared with controls were identified. A set of potential salt stress response and defense biomarkers was identified, such as cp31BHv, betaine-aldehyde dehydrogenase, leucine aminopeptidase 2, Cu/Zn superoxide dismutase, and 2-Cys peroxiredoxin BAS1, which could lead to a better understanding of the molecular basis of salt response and defense in food crops.
Soil salinity reduces the yield of the major crops, which is one of the severest problems in irrigated agriculture worldwide. However, how crops response and defense during different levels of salt treatment and recovery processes is still unclear, especially at the post-translational modification level. T. monococcum is a useful model for common wheat. Thus, proteomic and phosphoproteomic analyses of T. monococcum leaves were performed in our study, which provided novel insights into the underlying salt response and defense mechanisms in wheat and other crops.
盐度是影响作物产量和生产力的主要非生物胁迫因素。一粒小麦与乌拉尔图小麦（A（U）A（U））密切相关，乌拉尔图小麦被用作小麦A基因组研究的模式植物。在此，重点研究了盐胁迫诱导的动态蛋白质组和磷酸化蛋白质组图谱。一粒小麦幼苗先用80至320mM不同浓度的NaCl处理48小时，然后在进行蛋白质组和磷酸化蛋白质组分析之前进行48小时的恢复过程。结果，通过基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF-MS）从二维凝胶中鉴定出总共81个与盐胁迫和恢复相对应的斑点。这些蛋白质主要参与调节、胁迫防御、蛋白质折叠/组装/降解、光合作用、碳水化合物代谢、能量产生和运输、蛋白质代谢以及细胞结构。使用Pro-Q Diamond染色检测磷酸化蛋白质。最后，鉴定出20个在盐处理或恢复过程中与对照相比具有不同磷酸化水平的斑点。鉴定出一组潜在的盐胁迫响应和防御生物标志物，如cp31BHv、甜菜碱醛脱氢酶、亮氨酸氨肽酶2、铜/锌超氧化物歧化酶和2-半胱氨酸过氧化物酶BAS1，这可能有助于更好地理解粮食作物中盐响应和防御的分子基础。
土壤盐度降低了主要作物的产量，这是全球灌溉农业中最严重的问题之一。然而，作物在不同水平的盐处理和恢复过程中如何响应和防御仍不清楚，尤其是在翻译后修饰水平。一粒小麦是普通小麦的有用模式植物。因此，我们对一粒小麦叶片进行了蛋白质组和磷酸化蛋白质组分析，这为小麦和其他作物潜在的盐响应和防御机制提供了新的见解。
