Sarkari Bahador, Bavarsad Ahmadpour Niloofar, Moshfe Abdolali, Hajjaran Homa
Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Iran J Parasitol. 2016 Jan-Mar;11(1):126-30.
We describe a case of visceral leishmaniasis (VL) due to Leishmania tropica in a 50-year-old Iranian man lived in a VL-endemic area in southwest of Iran. The patient presented with a 3-month history of fever and splenomegaly. Clinical signs and serological findings were suggestive of VL. Spleen biopsy was taken from the patient and intracellular forms of Leishmania amastigotes was seen in Giemsa stained smears. The patient was treated with pentavalent antimonial compound with complete resolution of his systemic signs and symptoms. DNA was extracted from the microscopic slides of the spleen biopsy and the nagt (N-Acetylglucosamine-1-Phosphate Transferase) gene of Leishmania was PCR-amplified. Sequence analysis of the PCR product demonstrated that the case has 99% identity with those of available sequences of L. tropica. Intra-species variation within isolate was 0-0.1%; whereas, inter-species differences of the isolate with those of L. major and L. infantum was significantly higher.
我们描述了一例由热带利什曼原虫引起的内脏利什曼病（VL），患者为一名50岁的伊朗男子，居住在伊朗西南部的VL流行地区。患者有3个月的发热和脾肿大病史。临床症状和血清学检查结果提示为VL。对患者进行了脾脏活检，在吉姆萨染色涂片中可见利什曼原虫无鞭毛体的细胞内形态。患者接受了五价锑化合物治疗，全身症状和体征完全缓解。从脾脏活检的显微镜载玻片上提取DNA，并对利什曼原虫的nagt（N-乙酰葡糖胺-1-磷酸转移酶）基因进行PCR扩增。PCR产物的序列分析表明，该病例与热带利什曼原虫的现有序列有99%的同一性。分离株内的种内变异为0-0.1%；而该分离株与硕大利什曼原虫和婴儿利什曼原虫的种间差异明显更高。
