Mohebali M, Malmasi A, Hajjaran H, Jamshidi S, Akhoundi B, Rezaei M, Janitabar S, Zarei H, Charehdar S
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2011 Jun;6(2):69-73.
A 5-month old puppy with muco-cutaneous lesions in the chin, around lips and eyes was examined physically and microscopically for leishmaniasis. Muco-cutaneous lesions containing a large number of amastigotes of Leishmania spp. were observed. Amastigotes were also detected in liver and spleen of the puppy. The animal was positive with Dipstick rK39 kit and high level of anti-Leishmania antibodies was detected by direct agglutination test (DAT). DNA, Using PCR-RFLP technique extracted from cultured Leishmania promastigotes and L. tropica was identified. This is the first report of concurrent mucosal and visceral involvement of L. tropica in a puppy from Iran.
对一只5个月大、下巴、嘴唇周围和眼睛出现黏膜皮肤病变的幼犬进行了利什曼病的体格检查和显微镜检查。观察到黏膜皮肤病变中含有大量利什曼原虫属的无鞭毛体。在幼犬的肝脏和脾脏中也检测到了无鞭毛体。该动物用Dipstick rK39试剂盒检测呈阳性，通过直接凝集试验（DAT）检测到高水平的抗利什曼原虫抗体。利用PCR-RFLP技术从培养的利什曼原虫前鞭毛体中提取DNA并鉴定出热带利什曼原虫。这是伊朗一只幼犬同时出现热带利什曼原虫黏膜和内脏感染的首次报告。
