Brzóska Malgorzata M, Rogalska Joanna, Roszczenko Alicja, Galazyn-Sidorczuk Malgorzata, Tomczyk Michal
Department of Toxicology, Medical University of Bialystok, Bialystok, Poland.
Department of Pharmacognosy, Medical University of Bialystok, Bialystok, Poland.
Planta Med. 2016 May;82(7):621-31. doi: 10.1055/s-0042-103593. Epub 2016 Apr 20.
Recently, we demonstrated in a rat model that consumption of a polyphenol-rich extract obtained from the berries of Aronia melanocarpa could protect from cadmium-induced disorders in bone turnover and changes in bone mineral status. The aim of this study was to investigate whether the osteoprotective effect of this extract is mediated by the oxidative defense system. Enzymatic and nonenzymatic antioxidants, total antioxidative and oxidative status, hydrogen peroxide, and markers of oxidative protein, lipid, and DNA damage were determined in bone tissue at the distal femoral epiphysis of female Wistar rats receiving 0.1 % aqueous A. melanocarpa extract (prepared from the lyophilized commercial extract containing 65.74 % of polyphenols) as the only drinking fluid and/or cadmium in the diet (1 and 5 mg/kg) for 3, 10, 17, and 24 months. The total oxidative and antioxidative status of the serum was also evaluated. The administration of A. melanocarpa extract provided significant protection from cadmium-induced oxidative stress in the bone and serum, and from lipid peroxidation and oxidative damage to the protein and DNA in the bone tissue. Numerous correlations were noted between indices of the oxidative/antioxidative bone status and markers of bone metabolism previously assayed in the animals receiving A. melanocarpa extract. The results allow the conclusion that the ability of A. melanocarpa extract to mediate the oxidative defense system and prevent oxidative modifications of protein, lipid, and DNA in the bone tissue plays an important role in its osteoprotective action under exposure to cadmium. The findings provide further evidence supporting our suggestion that chokeberry may be a promising natural agent for protection against the toxic action of cadmium in women chronically exposed to this metal.
最近，我们在大鼠模型中证明，食用从黑果腺肋花楸浆果中提取的富含多酚的提取物，可以预防镉引起的骨转换紊乱和骨矿物质状态变化。本研究的目的是调查这种提取物的骨保护作用是否由氧化防御系统介导。在雌性Wistar大鼠股骨远端骨骺的骨组织中，测定了酶促和非酶促抗氧化剂、总抗氧化和氧化状态、过氧化氢以及氧化蛋白质、脂质和DNA损伤的标志物。这些大鼠以0.1%的黑果腺肋花楸水提取物（由含有65.74%多酚的冻干商业提取物制备）作为唯一饮用水和/或在饮食中添加镉（1和5mg/kg），持续3、10、17和24个月。同时还评估了血清的总氧化和抗氧化状态。黑果腺肋花楸提取物的给药显著保护了骨骼和血清免受镉诱导的氧化应激，以及骨组织中的脂质过氧化和蛋白质及DNA的氧化损伤。在接受黑果腺肋花楸提取物的动物中，氧化/抗氧化骨状态指标与先前测定的骨代谢标志物之间存在许多相关性。结果表明，黑果腺肋花楸提取物介导氧化防御系统以及防止骨组织中蛋白质、脂质和DNA氧化修饰的能力，在其对镉暴露的骨保护作用中起着重要作用。这些发现进一步证明了我们的观点，即黑果腺肋花楸可能是一种有前景的天然剂，可用于保护长期接触镉的女性免受镉的毒性作用。
