Hu X-S, Cheng D-L, Li K-J, Wang L-B, Yang X, Sun S, Wang Y-P, Li S-H, Lei Z-F, Zhang Z-Y
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Eur Rev Med Pharmacol Sci. 2016 Apr;20(7):1423-9.
The root of Helicteres angustifolia L. (Sterculiaceae) has been used as tea to treat diabetics effectively by local people in Laos. However, no scientific evidence is available for this ethnomedicinal usage. This study was undertaken to explore the hypoglycemic effect of aqueous extract from Helicteres angustifolia root.
The effect of aqueous extract from Helicteres angustifolia root on glucose consumption in C2C12 myotubes was investigated at a dose of 25, 50 and 100 µg/mL, respectively. The alpha-glucosidase inhibitory activity of the extract was evaluated using rat intestinal maltase and sucrose. Moreover, oral sucrose tolerance test (OSTT) in normal and streptozotocin induced diabetic rats was performed. Finally, their cytotoxicity in C2C12 cells and acute oral toxicity in rats was analyzed.
Aqueous root extract of Helicteres angustifolia significantly enhanced glucose consumption in C2C12 myotubes. The extract also significantly inhibited rat intestinal maltase (IC50 = 1.44 ± 0.24 mg/mL) and sucrase activity (IC50 = 0.54 ± 0.12 mg/mL), respectively. The OSTT results showed that the extract significantly suppressed the increase of blood glucose levels in normal and diabetic rats. The extract was also proven to have low acute toxicity (LD50 > 5 g/kg) and low cytotoxicity in C2C12 cells (IC50 > 0.4 mg/mL).
The findings from this study indicate that aqueous root extract of Helicteres angustifolia possesses significant alpha-glucosidase inhibitory activity and moderate enhanced glucose consumption activity, while with low cytotoxic and acute toxicity.
老挝当地人将狭叶山芝麻（梧桐科）的根用作茶来有效治疗糖尿病患者。然而，这种民族药用方法尚无科学依据。本研究旨在探究狭叶山芝麻根水提取物的降血糖作用。
分别以25、50和100μg/mL的剂量研究狭叶山芝麻根水提取物对C2C12肌管葡萄糖消耗的影响。使用大鼠肠道麦芽糖酶和蔗糖评估该提取物的α-葡萄糖苷酶抑制活性。此外，对正常大鼠和链脲佐菌素诱导的糖尿病大鼠进行口服蔗糖耐量试验（OSTT）。最后，分析其对C2C12细胞的细胞毒性和对大鼠的急性经口毒性。
狭叶山芝麻根水提取物显著增强了C2C12肌管中的葡萄糖消耗。该提取物还分别显著抑制了大鼠肠道麦芽糖酶（IC50 = 1.44±0.24mg/mL）和蔗糖酶活性（IC50 = 0.54±0.12mg/mL）。OSTT结果表明，该提取物显著抑制了正常大鼠和糖尿病大鼠血糖水平的升高。该提取物还被证明具有低急性毒性（LD50>5g/kg）和对C2C12细胞的低细胞毒性（IC50>0.4mg/mL）。
本研究结果表明，狭叶山芝麻根水提取物具有显著的α-葡萄糖苷酶抑制活性和适度增强的葡萄糖消耗活性，同时具有低细胞毒性和急性毒性。
