Keeney M, Jiang X Y, Yamane M, Lee M, Goodman S, Yang F
Department of Orthopaedic Surgery, 300 Pasteur Dr., Edwards R105, Stanford, CA 94305, USA.
Program of Human Biology, Stanford University, Stanford, CA 94305, USA.
J Mater Chem B. 2015 Nov 7;3(45):8757-8770. doi: 10.1039/c5tb00450k. Epub 2015 Sep 16.
Since its introduction in the early 1990s, layer-by-layer (LbL) self-assembly of films has been widely used in the fields of nanoelectronics, optics, sensors, surface coatings, and controlled drug delivery. The growth of this industry is propelled by the ease of film manufacture, low cost, mild assembly conditions, precise control of coating thickness, and versatility of coating materials. Despite the wealth of research on LbL for biomolecule delivery, clinical translation has been limited and slow. This review provides an overview of methods and mechanisms of loading biomolecules within LbL films and achieving controlled release. In particular, this review highlights recent advances in the development of LbL coatings for the delivery of different types of biomolecules including proteins, polypeptides, DNA, particles and viruses. To address the need for co-delivery of multiple types of biomolecules at different timing, we also review recent advances in incorporating compartmentalization into LbL assembly. Existing obstacles to clinical translation of LbL technologies and enabling technologies for future directions are also discussed.
自20世纪90年代初被引入以来，逐层（LbL）自组装薄膜已广泛应用于纳米电子学、光学、传感器、表面涂层和可控药物递送等领域。该行业的发展得益于薄膜制造的简便性、低成本、温和的组装条件、涂层厚度的精确控制以及涂层材料的多功能性。尽管关于LbL用于生物分子递送的研究丰富，但临床转化一直有限且进展缓慢。本综述概述了在LbL薄膜中负载生物分子并实现控释的方法和机制。特别是，本综述重点介绍了用于递送不同类型生物分子（包括蛋白质、多肽、DNA、颗粒和病毒）的LbL涂层开发的最新进展。为满足在不同时间共同递送多种类型生物分子的需求，我们还综述了将分隔化纳入LbL组装的最新进展。还讨论了LbL技术临床转化的现有障碍以及未来方向的使能技术。
