Department of Chemistry and ‡Markey Center for Structural Biology and Department of Biological Sciences, Purdue University , West Lafayette, Indiana 47907, United States.
J Am Chem Soc. 2015 Feb 11;137(5):1730-3. doi: 10.1021/ja5101307. Epub 2015 Jan 28.
Here we report the assembly of multilayered DNA nanocages. The layers can be separated in response to a chemical cue, ATP. This is an effort to increase the structural complexity of DNA nanocages. The structures have been characterized by native polyacrylamide gel electrophoresis, atomic force microscopy, and cryogenic electron microscopy. We envision that the layer-by-layer assembly strategy used in this study can be easily applied to other DNA nanocages to form Russian-doll-like semisolid structures, while the chemically activated layer separation makes a contribution to the development of "smart" DNA nanocages.
在这里，我们报告了多层 DNA 纳米笼的组装。这些层可以响应化学信号（ATP）进行分离。这是为了提高 DNA 纳米笼的结构复杂性。这些结构已经通过天然聚丙烯酰胺凝胶电泳、原子力显微镜和低温电子显微镜进行了表征。我们设想，本研究中使用的层层组装策略可以很容易地应用于其他 DNA 纳米笼，以形成类似俄罗斯套娃的半固态结构，而化学激活的层分离为“智能”DNA 纳米笼的发展做出了贡献。
