NONRATT021972长链非编码RNA干扰对氧糖剥夺后P2X7受体介导的PC12神经元损伤的影响
The effects of NONRATT021972 lncRNA siRNA on PC12 neuronal injury mediated by P2X7 receptor after exposure to oxygen-glucose deprivation.
作者信息
Li Guilin, Zou Lifang, Xie Wei, Wen Shiyao, Xie Qiuyu, Gao Yun, Xu Changshui, Xu Hong, Liu Shuangmei, Wang Shouyu, Xue Yun, Wu Bing, Lv Qiulan, Ying Mofeng, Zhang Xi, Liang Shangdong
机构信息
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Undergraduate student of grade 2012, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
出版信息
Purinergic Signal. 2016 Sep;12(3):479-87. doi: 10.1007/s11302-016-9513-8. Epub 2016 Apr 21.
Adenosine triphosphate (ATP) participates in signal transmission by acting on P2X receptors, and the P2X7 receptor is involved in the pathophysiological changes of ischemic injury. The PC12 cell line is a popular model system to study sympathetic neuronal function. Long noncoding RNAs (lncRNAs) are highly expressed in the nervous system and serve as regulatory RNAs. In this study, the effects of NONRATT021972 lncRNA siRNA on P2X7-mediated PC12 neuronal injury after exposure to oxygen-glucose deprivation (OGD) were investigated. Our results showed that the viability of PC12 cells cultured with OGD or the P2X7 agonist BzATP was significantly decreased. Treatment with NONRATT021972 siRNA reversed the decreased viability of PC12 cells under OGD conditions. The upregulated P2X7 mRNA and protein levels in PC12 cells under OGD conditions or BzATP treatment were significantly decreased when pretreated with NONRATT021972 siRNA. Moreover, NONRATT021972 siRNA treatment effectively suppressed the increase in [Ca(2+)]i induced by OGD or P2X7 agonists (ATP or BzATP) in PC12 cells. Therefore, treatment with NONRATT021972 siRNA may decrease sympathetic neuronal injury induced by ischemia.
三磷酸腺苷（ATP）通过作用于P2X受体参与信号传递，且P2X7受体参与缺血性损伤的病理生理变化。PC12细胞系是研究交感神经元功能常用的模型系统。长链非编码RNA（lncRNA）在神经系统中高度表达并作为调控RNA发挥作用。在本研究中，研究了NONRATT021972 lncRNA siRNA对氧糖剥夺（OGD）后P2X7介导的PC12神经元损伤的影响。我们的结果显示，用OGD或P2X7激动剂BzATP培养的PC12细胞活力显著降低。用NONRATT021972 siRNA处理可逆转OGD条件下PC12细胞活力的降低。当用NONRATT021972 siRNA预处理时，OGD条件下或BzATP处理的PC12细胞中上调的P2X7 mRNA和蛋白水平显著降低。此外，NONRATT021972 siRNA处理有效抑制了OGD或P2X7激动剂（ATP或BzATP）诱导的PC12细胞中[Ca(2+)]i的增加。因此，用NONRATT021972 siRNA处理可能会减轻缺血诱导的交感神经元损伤。
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