Su Yulong, Wang Qian, Zhang Chengsheng, Gu Jingang, Shi Sujuan, Manik S M Nuruzzaman, Mao Jingjing, Li Shigui, Lei Qiang, Wu Renjun, Yin Ying, Qu Jiankang, Li Liang, Liu Haobao
Wei Sheng Wu Xue Bao. 2015 Dec 4;55(12):1543-50.
The aim of this study was to screen tobacco straw and nicotine degrading microorganism.
The bacterium was isolated from tobacco field soil using medium containing tobacco straw as the sole carbon and nitrogen source. We identified the bacterium through morphological and physiological characterization combined with the result of 16S rRNA gene sequence and data analysis. We also studied the lignocelluloses degradation and enzyme activities related to the degradation of lignin and cellulose in liquid state fermentation of tobacco stalk.
The bacterium was identified as Bacillus megaterium and we had demonstrated that it has a good ability to degrade lignin in tobacco straw when fermented in liquid state. It showed the highest laccase production of 418. 52 U/L while the highest lignin peroxides and manganese peroxides activity was 19. 71 U/L and 64. 71 U/L. On the other hand, we also found that nicotine in tobacco stem was totally degraded 20 d after inoculation.
to the isolated Bacillus megaterium is capable of degrading tobacco straw partially and nicotine totally.
本研究旨在筛选降解烟草秸秆和尼古丁的微生物。
以含烟草秸秆作为唯一碳源和氮源的培养基，从烟草田土壤中分离细菌。通过形态学和生理学特征结合16S rRNA基因序列及数据分析结果对该细菌进行鉴定。同时，研究了烟草秸秆液态发酵过程中木质纤维素的降解情况以及与木质素和纤维素降解相关的酶活性。
该细菌被鉴定为巨大芽孢杆菌，且已证明其在液态发酵时具有良好的降解烟草秸秆中木质素的能力。其漆酶产量最高达418.52 U/L，而木质素过氧化物酶和锰过氧化物酶的最高活性分别为19.71 U/L和64.71 U/L。另一方面，我们还发现接种后20天烟草茎中的尼古丁被完全降解。
分离得到的巨大芽孢杆菌能够部分降解烟草秸秆并完全降解尼古丁。
