Zhang Lebin, An Jin, Li Lijuan, Wang Hengwei, Liu Dawen, Li Ning, Cheng Hairong, Deng Zixin
Innovation & Application Institute, Zhejiang Ocean University , Zhoushan 316022, China.
J Agric Food Chem. 2016 May 18;64(19):3828-37. doi: 10.1021/acs.jafc.6b00115. Epub 2016 May 9.
Currently, fructooligosaccharides (FOS) are industrially transformed from sucrose by purified enzymes or fungi cells. However, these methods are expensive and time-consuming. An economical approach to producing FOS using erythritol-producing yeast cells was described in this study. Fructosyltransferase from Aspergillus oryzae was displayed on the cell surface of Yarrowia lipolytica, resulting in an engineered strain capable of transforming sucrose to FOS. An amount of 480 g/L FOS was produced within 3 h in a solution of 800 g/L sucrose and 5 g/L cells (dry cell weight, DCW) at pH 6.0 and 60 °C, with a yield of 60% of total sucrose and a productivity of 160 g/(L·h). The yeast pastes from the erythritol industry can be repeatedly used as the whole-cell catalysts at least 10 times by this newly developed approach. This efficient method is attractive for the large-scale production of FOS from sucrose.
目前，低聚果糖（FOS）是通过纯化酶或真菌细胞从蔗糖工业转化而来的。然而，这些方法成本高且耗时。本研究描述了一种使用产赤藓糖醇的酵母细胞生产FOS的经济方法。来自米曲霉的果糖基转移酶展示在解脂耶氏酵母的细胞表面，从而产生一种能够将蔗糖转化为FOS的工程菌株。在pH 6.0和60°C条件下，在含有800 g/L蔗糖和5 g/L细胞（干细胞重量，DCW）的溶液中，3小时内可产生480 g/L的FOS，蔗糖总产率为60%，生产效率为160 g/(L·h)。通过这种新开发的方法，赤藓糖醇工业生产的酵母糊可作为全细胞催化剂重复使用至少10次。这种高效方法对于从蔗糖大规模生产FOS具有吸引力。
