Mudurangaplar Bharath, Peerapur B V
PhD Scholar, Department of Microbiology, Shri B.M. Patil Medical College and Research Center, BLDE University , Bijapur, Karnataka, India .
Professor and HOD, Department of Microbiology, Raichur Institute of Medical Sciences (RIMS) , Raichur, Karnataka, India .
J Clin Diagn Res. 2016 Mar;10(3):DC14-7. doi: 10.7860/JCDR/2016/18370.7509. Epub 2016 Mar 1.
Indian ocean islands and India have experienced massive severe Chikungunya outbreak from 2005 up till now and then Chikungunya became epidemic in India. The mutations that occurred in E1 gene were responsible for increased infectivity, virulence and host adaptability. It is important to find out the genotype and its probable evolvement and novel mutations in the E1 gene reported during 2006-2009 from the current isolates, which may affect the local protein structure.
To perform Molecular diagnosis and Molecular Characterisation of Chikungunya virus isolates.
A total of 33 samples were included in the study. RNA was isolated from 33 serum samples and Real time PCR was carried out. Further, Nested PCR and E1 partial gene sequencing was performed. Phylogenetic analysis, mutational analysis and protein modelling studies were carried out.
Out of 33 samples tested, 31 were found positive for CHIK RNA. Phylogenetic analysis showed that isolates belongs to ECSA genotype and E1K211E, E1M269V and E1D284E mutations were observed from all the isolates.
The isolates may have evolved from ECSA Reunion island strains and identified unique mutations in E1 gene were maintained. These mutations have not affected local protein structure.
从2005年至今，印度洋岛屿和印度经历了大规模严重的基孔肯雅热疫情爆发，随后基孔肯雅热在印度流行开来。E1基因发生的突变导致了传染性、毒力和宿主适应性的增强。从当前分离株中找出2006 - 2009年期间报道的E1基因的基因型及其可能的演变和新突变很重要，因为这些可能会影响局部蛋白质结构。
对基孔肯雅病毒分离株进行分子诊断和分子特征分析。
本研究共纳入33个样本。从33份血清样本中分离RNA并进行实时聚合酶链反应（Real time PCR）。此外，进行巢式聚合酶链反应（Nested PCR）和E1部分基因测序。开展系统发育分析、突变分析和蛋白质建模研究。
在检测的33个样本中，31个样本的基孔肯雅病毒核糖核酸（CHIK RNA）呈阳性。系统发育分析表明，分离株属于东中非和南非基因型（ECSA genotype），并且在所有分离株中均观察到E1K211E、E1M269V和E1D284E突变。
这些分离株可能是从东中非和南非留尼汪岛毒株进化而来，并且在E1基因中鉴定出的独特突变得以保留。这些突变并未影响局部蛋白质结构。
