Vichi P, Tritton T R
Department of Pharmacology, University of Vermont, College of Medicine, Burlington 05405.
Cancer Res. 1989 May 15;49(10):2679-82.
Adriamycin causes a variety of biological actions and is an effective cytotoxic agent against proliferating cells. In this paper we show that the drug is not limited in its action solely to cytotoxicity, but can also stimulate cell growth under the appropriate conditions. Using the survival assay of cloning in soft agar, we present data showing that the conditions for Adriamycin-induced growth stimulation are that the drug be in a subtoxic concentration range of 10(-10)-10(-9) M (greater than 10(-8) M causes cytotoxicity) and that the growth medium be suboptimal. This latter condition is satisfied by either growing cells for an extended period in order to exhaust the growth supporting capacity of the medium, or by growing the cells at low (less than 10%) serum concentrations. Several active anthracycline congeners also have the ability to stimulate growth. The results indicate that the cytotoxic anticancer agent Adriamycin can stimulate the proliferation of some cells.
阿霉素具有多种生物学作用，是一种针对增殖细胞的有效细胞毒性药物。在本文中，我们表明该药物的作用不仅限于细胞毒性，在适当条件下还能刺激细胞生长。通过软琼脂克隆存活试验，我们提供的数据表明，阿霉素诱导生长刺激的条件是药物处于10(-10)-10(-9)M的亚毒性浓度范围（大于10(-8)M会导致细胞毒性），且生长培养基为次优状态。后一种条件可通过以下两种方式实现：一是让细胞长时间生长以耗尽培养基的生长支持能力，二是在低血清浓度（低于10%）下培养细胞。几种活性蒽环类同系物也具有刺激生长的能力。结果表明，细胞毒性抗癌药物阿霉素可以刺激某些细胞的增殖。
