Scott Charlotte L, Wright Pamela B, Milling Simon W F, Mowat Allan McI
Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK.
Laboratory of Mucosal Immunology and Immunoregulation, Inflammation Research Centre (IRC), VIB Ghent University, Ghent (Zwijnaarde), Belgium.
Methods Mol Biol. 2016;1423:101-18. doi: 10.1007/978-1-4939-3606-9_7.
The identification of conventional dendritic cells (cDCs) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from other intestinal mononuclear phagocytes such as macrophages (MFs). Here we detail the protocols we have developed to isolate live leukocytes from both murine and human small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs in such preparations.
从肠道中分离细胞存在困难，且重叠的标志物使得准确区分常规树突状细胞（cDC）与其他肠道单核吞噬细胞（如巨噬细胞，MF）变得复杂，这阻碍了肠道黏膜中cDC的鉴定。在此，我们详细介绍我们开发的从鼠类和人类小肠及大肠中分离活白细胞的方案，并描述可用于在此类样本中鉴定真正cDC的可靠策略。
