Section of Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO, USA.
Gastrointestinal Eosinophilic Diseases Program (GEDP) and Digestive Health Institute (DHI), Children's Hospital Colorado, Aurora, CO, USA.
Methods Mol Biol. 2021;2241:243-255. doi: 10.1007/978-1-0716-1095-4_19.
Eosinophils are primarily tissue-dwelling leukocytes. Utilization of flow cytometry techniques applied to digested tissues is expanding the scope of organs within which eosinophils are identified at baseline and is providing deeper insights into categorizing phenotypically and functionally distinct tissue-resident eosinophil subpopulations in health and disease. Here we describe a tissue digestion protocol and flow cytometry gating strategy for identification and isolation of tissue eosinophils from the small intestine of mice. This protocol is also amenable to the isolation and characterization of colonic eosinophils, and of intestinal eosinophils from human resected tissues.
嗜酸性粒细胞主要是组织驻留的白细胞。应用于消化组织的流式细胞术技术的利用正在扩大在基线时鉴定嗜酸性粒细胞的器官范围，并为健康和疾病中分类表型和功能不同的组织驻留嗜酸性粒细胞亚群提供更深入的见解。在这里，我们描述了从小鼠小肠中鉴定和分离组织嗜酸性粒细胞的组织消化方案和流式细胞术门控策略。该方案还适用于分离和表征结肠嗜酸性粒细胞以及从人切除组织中分离和表征肠嗜酸性粒细胞。
