采用HIV检测方法评估口腔截获标本采集装置与配对血清/血浆标本的效果。
Evaluation of the intercept oral specimen collection device with HIV assays versus paired serum/plasma specimens.
作者信息
Beelaert G, Van Heddegem L, Van Frankenhuijsen M, Vandewalle G, Compernolle V, Florence E, Fransen K
机构信息
Department of Clinical Science, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium.
Blood Service, Belgian Red Cross-Flanders, Mechelen, Belgium.
出版信息
J Virol Methods. 2016 Aug;234:164-8. doi: 10.1016/j.jviromet.2016.04.014. Epub 2016 Apr 30.
Oral fluid has many advantages over blood-based techniques: it is less invasive, eliminates the occupational risk associated with needle stick accidents and collection can be self-administrated. Each individual test is packaged with a corresponding collection device. This study tested the suitability of the Intercept Oral Specimen Collection Device for different HIV diagnostic tests: three different rapid HIV tests and two adapted ELISAs, which were evaluated and compared with a gold standard on blood. In addition a total IgG quantification was performed to demonstrate the quality of the specimen. HIV antibodies were detected with a sensitivity of 100%, 99.3%, 98.6%, 100% and 95.7% for, DPP, OraQuick, Aware, Genscreen and Vironostika respectively using the Intercept Collection Device. Respective specificities were 100%, 100%, 99.3%, 97.3% and 100%.
与基于血液的检测技术相比，口腔液具有诸多优势：它侵入性较小，消除了与针刺事故相关的职业风险，且采集过程可自行操作。每项单独检测都配有相应的采集装置。本研究测试了Intercept口腔样本采集装置对不同HIV诊断检测的适用性：三种不同的HIV快速检测以及两种改良的酶联免疫吸附测定法（ELISA），并将其与血液检测的金标准进行评估和比较。此外，还进行了总IgG定量分析以证明样本质量。使用Intercept采集装置检测HIV抗体时，DPP、OraQuick、Aware、Genscreen和Vironostika的灵敏度分别为100%、99.3%、98.6%、100%和95.7%。各自的特异性分别为100%、100%、99.3%、97.3%和100%。
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