Department of Serology Research and Development, Curative, Monrovia, CA, United States.
Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, United States.
Front Immunol. 2021 Sep 3;12:701411. doi: 10.3389/fimmu.2021.701411. eCollection 2021.
In March 2020, the World Health Organization (WHO) declared a global health emergency-the coronavirus disease 2019 (COVID-19) pandemic. Since then, the development and implementation of vaccines against the virus amidst emerging cases of re-infection has prompted researchers to work towards understanding how immunity develops and is sustained. Serological testing has been instrumental in monitoring the development and persistence of antibodies against SARS-CoV-2 infection, however inconsistencies in detection have been reported by different methods. As serological testing becomes more commonplace, it is important to establish widespread and repeatable processes for monitoring vaccine efficacy. Therefore, we present enzyme linked immunosorbent assays (ELISAs) compatible for antibody detection in saliva as highly accurate, efficacious, and scalable tools for studying the immune response in individuals vaccinated against SARS-CoV-2.
2020 年 3 月，世界卫生组织（WHO）宣布全球卫生紧急事件——2019 年冠状病毒病（COVID-19）大流行。此后，针对病毒的疫苗的开发和应用，以及新出现的再感染病例，促使研究人员努力了解免疫是如何产生和维持的。血清学检测在监测针对 SARS-CoV-2 感染的抗体的发展和持续方面发挥了重要作用，然而不同方法的检测结果存在不一致性。随着血清学检测变得更加普遍，建立广泛和可重复的监测疫苗效果的流程非常重要。因此，我们提出了与唾液中抗体检测兼容的酶联免疫吸附测定（ELISA），作为研究 SARS-CoV-2 疫苗接种个体免疫反应的高度准确、有效和可扩展的工具。
