Nikolov J T, Dimitrov O A, Kabaivanova S D, Ivanova V L, Moskova V V
Department of Human and Animal Physiology, Faculty of Biology, University of Sofia, Bulgaria.
Endocrinol Exp. 1989 Mar;23(1):29-35.
The effect of estradiol on phosphorylation with gamma-32P-ATP of ribosome proteins from polyribosome fraction, isolated from female rat liver is found out. Rats (180 g) used were: sham-operated, ovariectomized and treated after ovariectomy for 11 days with 20 micrograms 17 beta-estradiol-dipropionate per 100 g body weight. The estradiol deficiency after ovariectomy decreased phosphorylation, but the estrogenization of ovariectomized rats increases it twice. In vitro the hormone (1-2 mumol/l) [corrected] also stimulated ribosome protein phosphorylation. It is supposed that the hormone may take part in the regulation of protein synthesis in the translational region of the process.
研究了雌二醇对从雌性大鼠肝脏分离的多核糖体组分中核糖体蛋白与γ-32P-ATP磷酸化的影响。所用大鼠（180克）为：假手术组、卵巢切除组以及卵巢切除后用每100克体重20微克17β-雌二醇二丙酸酯处理11天的组。卵巢切除后的雌二醇缺乏降低了磷酸化，但卵巢切除大鼠的雌激素化使其增加了两倍。在体外，该激素（1-2微摩尔/升）[校正后]也刺激核糖体蛋白磷酸化。推测该激素可能参与该过程翻译区域的蛋白质合成调节。
