Dimitrov O A, Nikolov Y T, Kabaivanova S D, Moskova V V, Ivanova V L
Acta Physiol Pharmacol Bulg. 1985;11(4):74-9.
The article studies the effect of 17, beta-estradiol on the binding of cytosol estradiol-receptor complex with chromatin isolated from the liver of female albino rats (sham-operated, ovariectomized and treated after the ovariectomy with 20 micron hormone/100 g body weight for 11 days) and sexually mature male albino rats. Higher binding of the complex is found for the female animals compared with the males. Parallel experiments are made to study the incorporation of 3H-thymidine and 14C-glycine in vivo, in acid-soluble and acid-insoluble liver fractions of female rats, respectively, which is used as a basis for assessing the rate of DNA and protein synthesis. Ovariectomy induces considerable reduction of the binding of the estradiol-receptor complex with chromatin, as well as of the DNA and protein synthesis. Hormonal treatment of the ovariectomized rats is followed by substantial increase in the parameters investigated above the level measured in intact animals. The observed effects of the hormone on the liver correspond to the accepted view concerning the action of steroid sex hormones on their target organs.
本文研究了17β-雌二醇对雌性白化大鼠（假手术组、去卵巢组以及去卵巢后用20微克激素/100克体重处理11天的组）和性成熟雄性白化大鼠肝脏中分离出的染色质上细胞溶质雌二醇-受体复合物结合的影响。结果发现，与雄性动物相比，雌性动物的复合物结合率更高。同时进行了平行实验，分别研究3H-胸腺嘧啶核苷和14C-甘氨酸在雌性大鼠肝脏的酸溶性和酸不溶性部分的体内掺入情况，以此作为评估DNA和蛋白质合成速率的依据。去卵巢导致雌二醇-受体复合物与染色质的结合以及DNA和蛋白质合成显著减少。对去卵巢大鼠进行激素处理后，上述参数大幅增加，超过了完整动物所测水平。观察到的激素对肝脏的影响与关于类固醇性激素对其靶器官作用的公认观点相符。
