非洲热带树种（豆科，Detarioideae亚科）微卫星标记的特征分析
Characterization of microsatellite markers in the African tropical tree species (Fabaceae, Detarioideae).
作者信息
Tosso Félicien, Doucet Jean-Louis, Migliore Jérémy, Daïnou Kasso, Kaymak Esra, Kameni Franck S Monthe, Hardy Olivier J
机构信息
Central African Forests, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, B-5030 Gembloux, Belgium.
Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium.
出版信息
Appl Plant Sci. 2017 Jul 26;5(7). doi: 10.3732/apps.1700023. eCollection 2017 Jul.
PREMISE OF THE STUDY
Microsatellite primers (simple sequence repeats [SSRs]) were developed in (Fabaceae, Detarioideae) to study population genetic structure and the history of African vegetation.
METHODS AND RESULTS
We isolated 18 polymorphic SSRs from a nonenriched genomic library. This set of primer pairs was tested on four populations, and the results showed two to 16 alleles per locus with mean observed and expected heterozygosities of 0.27 ± 0.05 and 0.57 ± 0.05, respectively. Cross-amplification tests in 13 congeneric species were successful for the four taxa belonging to the subgenus .
CONCLUSIONS
This set of microsatellite markers will be useful to investigate the phylogeography and population genetics of , a key representative of African semideciduous moist forests.
研究前提
开发了豆科云实亚科的微卫星引物（简单序列重复序列[SSRs]），用于研究种群遗传结构和非洲植被历史。
方法与结果
我们从一个未富集的基因组文库中分离出18个多态性SSR。这组引物对在四个种群上进行了测试，结果显示每个位点有2至16个等位基因，平均观察杂合度和期望杂合度分别为0.27±0.05和0.57±0.05。在13个同属物种中的交叉扩增测试对属于该亚属的四个分类群成功。
结论
这组微卫星标记将有助于研究非洲半落叶湿润森林的关键代表物种的系统地理学和种群遗传学。
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